摘要
分析柿单宁与蛋白质和卵磷脂等在模拟胃液环境中的絮凝趋势,探讨胃柿石的形成原因。以牛心柿的生柿、脱涩硬柿和后熟软甜柿为试材,提取并测定其总、游离和缩合单宁含量;选取美国药典的模拟胃液配方,采用透射比浊法,对比分析生柿、熟软柿中单宁与模拟胃液,及与含有蛋白质、卵磷脂的模拟胃液作用产生的絮凝趋势。结果表明:生柿游离单宁的含量(7.076g/L)高于脱涩硬柿(3.962g/L)和后熟软柿(3.679g/L),其缩合单宁的含量(4.002g/L)低于脱涩硬柿(7.392g/L)和后熟软柿(7.538g/L);生柿、熟软柿中单宁与模拟胃液作用均有较强絮凝趋势,它们与含有蛋白质或卵磷脂的模拟胃液作用可产生更强的絮凝趋势;参试物絮凝趋势从强到弱依次为明胶>牛血清>卵磷脂>干酪素,生柿>熟软柿和脱涩硬果。柿果形成胃柿石的主要原因是其单宁含量较高,并与摄入不当(空腹、同时摄入明胶或牛血清类蛋白质)有关。
In order to understand the mechanism of gastrolithiasis formation,the flocculation trend of the persimmon tannin with casein and bovine serum albumin, gelatin, and lecithin in simulated gastric fluid was investigated. After step-extracting their free and condensed tannins and analyzing the con tents of them, the flocculation extents （expressed as turbidity） of the raw persimmon and ripe persim mon tannins with protein and lecithin in simulated gastric fluid were analyzed respectively using the simulated gastric fluid documented in the United States Pharmacopoeia in 1995. Moreover, we com pared the flocculation trend of raw persimmon and ripe persimmon tannins by turbidimetry method. The free tannins content of raw persimmon（7. 076 g/L） was higher than that of deastringented per simmon（3. 962 g/L）and ripe persimmon（3. 679 g/L）. However, the condensed tannin content of raw persimmon （4. 002 g/L）） was lower than that of deastringented persimmon（7. 392 g/L） and ripe per simmon（7. 538 g/L）. The flocculation trend of persimmon tannin with protein and lecithin in simula ted gastric fluid, and the persimmon tannin directly interacting with the simulated gastric fluid were relatively high;among them, the flocculation trend of gelatin was the strongest among casein and bo-vine serum albumin（BSA） and lecithin. The flocculation trend of raw persimmon tannin was stronger than that of ripe persimmon tannins. The formation of gastrolithiasis may be caused by high concen tration persimmon tannin in persimmon, especially when taking it with empty stomach or taking it to-gether with lecithin and gelatin.
出处
《西北农业学报》
CAS
CSCD
北大核心
2014年第1期177-182,共6页
Acta Agriculturae Boreali-occidentalis Sinica
基金
国家科技支撑计划(2011BAD29B04)
关键词
柿单宁
模拟胃液
絮凝
比浊法
Persimmon tannin Simulated gastric fluid Flocculation Turbidimetry
