摘要
用60%乙醇超声提取猫眼草根、茎、叶不同部位中的总黄酮,并以芦丁为对照品,用紫外分光光度计在510 nm处测定不同药用部位中总黄酮的含量。结果表明,芦丁标准溶液浓度在12.5~50μg/mL与吸光度呈良好的线性关系,线性回归方程为Y=0.011 5X-0.023 5,r=0.999 4（n=5）,全草的平均回收率为99.60%,RSD为1.60%。测得猫眼草根、茎、叶中黄酮含量分别为20.30,16.32,23.53 mg/g,叶中的总黄酮含量比根、茎含量高。
Extracting the total flavonoids in different sections of Enphorbia lunulata Bge. by refluxing with ultrasonic-assisted 60% ethanol and using rutin as the reference substance,the total flavonoids in Enphorbia lunulata Bge. were determined by UV absorption at 510 nm. The results showed that the linearity of rutin was obtained within the range of 12. 5 ~ 50. 0 μg/mL（Y = 0. 011 5X0. 023 5,r = 0. 999 4）（n = 5）,the average recovery rate was 99. 60% and RSD was 1. 60%（n = 9）. The content of total flavonoids in Enphorbia lunulata Bge. from root was 20. 30 mg/g,branch was 16. 32 mg/g and leaf was 23. 53 mg/g. The content of total flavonoids in Enphorbia lunulata Bge. from leaf was higher than root and branch individual.
出处
《应用化工》
CAS
CSCD
2014年第1期187-188,191,共3页
Applied Chemical Industry
基金
西安医学院大学生基金项目(12DXS03)
关键词
猫眼草
总黄酮
紫外分光光度法
Enphorbia lunulata Bge.
total flavonoids
UV spectrophotomety
