摘要
应用循环伏安法、差分脉冲伏安法、控制电位电解法等电化学方法和紫外光谱法研究了粉防己碱在玻碳电极上的电化学行为及其与DNA的相互作用,并对相关电化学动力学参数进行考察。结果表明,粉防己碱在玻碳电极上发生了受扩散控制的不可逆氧化反应,其在玻碳电极上的电子转移数为2、质子转移数为2、电荷转移系数为0.62。粉防己碱的峰电流随着DNA的加入而降低,且峰电位发生正移,表明粉防己碱与DNA通过嵌插方式相互作用生成复合物,同时计算了两者反应的结合数以及结合常数,结果显示粉防己碱和DNA以1∶1结合形成粉防己碱-DNA复合物,结合常数为4.27×103。
The electrochemical behaviors of tetrandrine at glassy carbon electrode as well as its interaction with DNA were studied using cyclic voltammetry,differential pulse voltammetry,controlled potential electrolysis and ultraviolet-visible spectroscopy.The results indicated that the electrochemical oxidation of tetrandrine was an irreversible process and the electrode process was controlled by the diffusion step,and the parameters of electrochemical kinetics were investigated.The results showed that the electrode process of tetrandrine on glassy carbon electrode was two electrons and two hydrions transfered,and the electron transfer coefficient was 0.62.In the presence of DNA,the oxidation current of tetrandrine was decreased considerably,and the peak potential was shifted to the positive direction,which suggested that the binding of tetrandrine to DNA was via intercalation.The association constant（β） and stoichiometric coefficient（m） were calculated to be 4.27 × 103 and 1,respectively.
出处
《分析测试学报》
CAS
CSCD
北大核心
2014年第1期98-102,共5页
Journal of Instrumental Analysis
基金
安徽省教育厅自然科学基金项目(KJ2011Z220)
