摘要
制备了纳米银/壳聚糖（AgCS）与乙炔黑（AB）复合修饰电极（AgCS-AB/GCE）,用紫外可见分光光度法、扫描电镜法对AgCS进行表征,用交流阻抗法对不同电极进行表征,用循环伏安法（CV）研究了乙萘酚（β-N）在AgCS-AB/GCE上的循环伏安行为.结果表明,在pH6.5的PBS缓冲液中,β-N在该修饰电极上出现一明显氧化峰,在60～ 240 mV/s扫速范围内,β-N的氧化峰电流（Ipa）与对应扫速平方根（v1/2）呈良好的线性关系,表明β-N在该电极上的电化学过程为受扩散控制的不可逆过程.计算了电极过程的动力学参数,运用方波伏安法测定不同浓度β-N的方波伏安曲线,结果表明β-N的氧化峰电流与其浓度在3.0 ×10-7～ 2.0×10-4 mol/L范围内呈良好线性关系（r=0.996）,检出限（S/N =3）为1.24×10-7 mol/L,加标回收率为99％～105％.
A new biosensor was fabricated with sol of nanosilver/chitosan（sol-AgCS） and acetylene black（AB） to form a compound film on a glassy carbon（GCE） electrode.The AgCS was characterized by UV spectrometry and scanning electron microscopy（SEM）.The different GCEs were characterized by electrochemical impedance spectroscopy （EIS）.The electrochemical behaviors of ethyl naphthol（β-N） on AgCS-AB/GCE were investigated by cyclic voltammetry （CV）.The results showed that β-N exhibited a sensitive oxidation peak in pH 6.5 PBS buffer solution.The oxidation peak current（Ipa） was linear to v1/2 in the range of 60-240 mV/s,which suggested that the electrode process of β-N was controlled by diffusion.The reaction electronic numbers,diffusion coefficient and other important parameters ofβ-N were calculated.The different concentrations ofβ-N were determined by square wave voltammetry,and a good linearity between peak current and concentration of β-N was observed in the range of 3.0 × 10-7-2.0 × 10-4 mol/L.The detection limit（S/N =3） was 1.24 × 10-7 mol/L and the spiked recoveries were in the range of 99％-105％.
出处
《分析测试学报》
CAS
CSCD
北大核心
2014年第1期88-92,97,共6页
Journal of Instrumental Analysis
基金
四川省高校科技创新团队建设项目(2010008)
关键词
乙萘酚
乙炔黑
纳米银
扫描电镜法
方波伏安法
ethyl naphthol
acetylene black
nano-silver
SEM
square wave voltammetry
