摘要
目的探讨PTEN基因在乳腺癌细胞中的表达及对细胞生物学行为的影响。方法选择PTEN正常表达的细胞株MDA—MB-231（M231），使用ShRNA方法沉默PTEN的表达，构建PTEN低表达的M231细胞株（M231-3001）及对照组细胞（M231-scr），用RT-PCR、Western—blot方法分析目的基因的表达抑制，通过CCK-8法、细胞划痕实验和Transwell细胞迁移实验等方法检测PTEN基因沉默后乳腺癌细胞株M231增殖、迁移及侵袭能力的改变。结果与对照组M231—8cr相比，M231—3001细胞株的PTENmRNA和蛋白的表达明显降低（P〈0．01），M231．3001细胞株相对于M231-scr细胞株增生、繁殖力增强，同时迁移和侵袭能力增加（均P〈0．05）。结论PTEN基因具有抑癌作用，其表达降低可促进癌细胞增生、迁移和侵袭。
Objective To investigate expression of the phosphatase and tensin homolog （PTEN） gene in breast cancer cell line and its effect on biologic characteristics. Methods The normal PTEN expression cell line MDA-MB-231 （M231） was used in this study. PTEN-shRNA plasmid was transected into M231 breast cancer cells to knock down the expression of PTEN. The changes of PTEN expression, proliferation,invasion and metastasis of PTEN knocked down cell were tested by RT-PCR, Western blot, CCK-8, scratch and Transwell. Results PTEN-shRNA was successfully transected into M231 cells. PTEN mRNA and protein expression was efficiently inhibited in M231-3001 cell lines than that in control group M231-scr（ P 〈 0. 01 ）, M231-3001 cell lines showed a greater capability of colony formation, migration and invasion than that in control group M231-scr （ all P 〈 0. 05 ）. Conclusions PTEN, as a suppression gene, its low expression can promote the proliferation, migration and invasion of breast cancer cells.
出处
《中华普通外科杂志》
CSCD
北大核心
2014年第1期49-53,共5页
Chinese Journal of General Surgery
基金
北京市卫生局科技成果和适宜技术推广项目（项目编号TG-2011-01）
