摘要
【目的】研究紫蓝草紫色素微波加热提取的最佳工艺条件,并探讨紫色素的耐氧化性能。【方法】以水为溶剂,通过单因素试验和正交试验考察微波功率、微波时间、料液比等因素对紫蓝草紫色素提取效果的影响。以空白紫色素溶液为对照,配制0.4%和1.0%的H2O2紫色素溶液,在不同时间段测定其吸光值,考察紫色素的耐氧化性。【结果】紫蓝草紫色素提取影响因素主次顺序为:微波功率>料液比>微波时间;最佳提取工艺条件为:微波功率320 W、微波时间80 s、料液比1∶20。紫色素加入H2O2放置72 h,吸光度变小,颜色变浅。【结论】微波水提技术提取紫蓝草紫色素的提取时间短,溶剂用量少,是提取紫蓝草紫色素的有效方法;紫色素有一定的耐氧化性。
【Objective】The best processing condition of extracting purpurin from Peristrophe roxburghiana by microwave heating was studied and the purpurin antioxidant performance was explored. 【Method】Taking water as dissolvent, single factor experiment and orthogonal test were used to observe the effects of microwave power, extracting time and ratio of solid to liquid on the result of extracting purpurin from Peristrophe roxburghiana. At the same time, making blank purpurin solution as control to prepare 0.4% and 1.0% hydrogen peroxide purpurin solution, and then the absorbency of hydrogen peroxide purpurin solution at different time was measured to examine the oxidation resistance of purpurin. 【Result】 The sequence of the factors affecting extraction of purpurin from Peristrophe roxburghiana was microwave power 〉 ratio of solid to liquid 〉 extracting time. The optimum process conditions were as follows： microwave power of 320 W, extracting time of 80 s and ratio of solid to liquid of 1∶20. After making the purpurin added with hydrogen peroxide standing for 72 h, its absorbency decreased and its color became shallow. 【Conclusion】Microwave-assisted water extraction technology could effectively extract purpurin from Peristrophe roxburghiana with short extraction duration and less solvent, so it was an effective method. Meanwhile, the purpurin possesses certain oxidation resistance.
出处
《南方农业学报》
CAS
CSCD
北大核心
2013年第12期2062-2065,共4页
Journal of Southern Agriculture
基金
国家自然科学基金项目(41163007)
广西教育厅科研项目(200911MS229)
关键词
紫蓝草
紫色素
微波水提
最佳工艺
耐氧化性
Peffstrophe roxburghiana
purpurin
microwave-assisted water extraction
optimum process conditions
oxidation resistance
