摘要
目的 观察天冬氨酸特异性半胱氨酸蛋白酶-3（Caspase-3）在重症急性胰腺炎（SAP）大鼠胰岛β细胞损伤中的作用.方法 雄性Wistar大鼠24只,体质量200-250 9,随机分为两组：假手术组（SO组）和重症急性胰腺炎模型组（SAP组）.大鼠禁食12h后采用胆胰管逆行注射5％牛磺胆酸钠制备SAP模型,造模后6h取血测定空腹血糖和血清胰岛素水平；随后腹腔注射20％葡萄糖液（2 g/kg）,30 min后再次抽血测定血糖和血清胰岛素水平.取胰尾部胰腺组织,电镜下观察大鼠胰岛β细胞超微结构变化,免疫组织化学分析胰岛β细胞Caspase-3表达.结果 两组空腹血糖差异无统计学意义（P＞0.05）,但是SAP组大鼠糖负荷后血糖明显高于SO组（P＜0.05）.糖负荷后,SO组大鼠血清胰岛素明显升高,而SAP组有所下降,两组差异有统计学意义（P＜0.05）；电镜下SAP组大鼠胰岛β细胞出现核固缩、染色质边集,线粒体、内质网、高尔基复合体肿胀,分泌颗粒减少等一系列超微结构病理改变,SAP组Caspase-3的表达较SO组增强,差异有统计学意义（P＜0.05）.结论 SAP大鼠血糖调控能力降低可能与胰岛β细胞Caspase-3表达增多有关,导致其超微结构的改变,从而影响胰岛素的分泌.
Objective To explore the effect of cysteinyl aspartate specific proteinase-3 （Caspase-3）on pancreatic beta cell damage in severe acute pancreatitis （SAP）.Methods Twenty-four male Wistar rats weighing 200-250 g were selected and randomly （random number） divided into the sham operation group （SO group） and SAP group （n =12 each）.The SAP model was induced by retrograde injection of 5% sodium taurocholate into the biliopancreatic duct.Rats were killed at 6 h after operation.The serum glucose （GLU） and plasma insulin （INS） levels were measured.After intraperitoneal injection of 20% glucose （2 g/kg） （a glucose load）,GLU and INS were measured 30 min later.The ultrastructure of the pancreatic beta cells was observed with an electron microscope.The expression of Caspase-3 was detected immunohistochemically.Results The serum glucose level 30 min after a glucose load and the expression of Caspase-3 were increased significantly in the SAP group as compared with the SO group （P 〈0.05）.The plasma insulin level was increased significantly 30 min after a glucose load in the SO group as compared with the SAP group （P 〈 0.05）.Pathological uhrastructural changes were found as follows：most pancreatic beta cells in the SAP group exhibited vacuole degeneration,the mitochondria had swolen and the rough endoplagmic reficuhm had expanded,and Caspase-3 expression was increased in the SAP group as compared with the SO group （P 〈 0.05）.Conclusion The increased beta cell Caspase-3 expression may reduce the blood glucose control ability of pancreatic beta cells in the SAP rats,leading to the ultrastructural changes,thus affecting the secretion of insulin.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2014年第1期41-43,共3页
Chinese Journal of Experimental Surgery
基金
湖北省自然科学基金资助项目（2010CDB06804）
