摘要
白血病是造血系统的恶性肿瘤,白血病细胞极易进入血液并浸润、损伤肝脏。本室既往研究表明,当归多糖(APS)能抑制白血病细胞的增殖和分化,但治疗白血病时对肝脏的影响未见报道。本研究通过尾静脉移植K562细胞建立人白血病NOD/SCID小鼠模型,通过腹腔注射APS、阿糖胞苷(Ara-c)以及两者联合(APS+Ara-c)治疗白血病小鼠,研究对肝脏的影响及其机制。结果表明,与白血病小鼠对照组相比,APS或Ara-c治疗后,外周血白细胞数显著降低;肝功能损伤减轻:丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)活性与总胆红素(TBiL)降低,白蛋白(Alb)升高;肝脏抗氧化能力下降得以抑制;抗氧化酶谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)活性升高,谷胱甘肽(GSH)、丙二醛(MDA)降低;炎症因子IL-1β,IL-6水平降低;肝指数减少;白血病细胞对肝小叶浸润减少,且凋亡率增加。APS+Ara-c联合治疗后肝脏病理恢复更加显著。综上所述,APS和Ara-c可减少肝脏内白血病细胞聚集,降低肝功能损伤和炎症因子水平,提高肝组织抗氧化能力,提示APS+Ara-c联合治疗可以发挥更好作用。
Leukemia is a type of malignant tumors of hematopoietic system with the abnormal increased immature leukemia cells showing metastasis and invasion ability. Liver is one of the main targets of the leukemia cells spread to, where they may continue to proliferate and differentiate and cause liver function damage, even liver failure. Our previous studies showed that Angelica polysscharides （APS）, the main effective components in Angelica sinensis of Chinese traditional medicine, was able to inhibit the proliferation and induced differentiation of the leukemia cells, however, its effect on the liver during the treatment remains elucidated. In the present study, the human leukemia NOD/SCID mouse model were established by implantation human leukemia K562 ceils line, then the leukemia mouse were treated with APS, Ara-c or APS ＋ Ara-c respectively by peritoneal injection for 14 days, to explore the effect and mechanism of the chemicals on the mouse liver. Compared to the human leukemia NOD/SCID mouse model group with the treatments of APS, Ara-c and APS ＋ Ara-c, We found that severe liver damage and pathological changes of the liver were able to alleviate ： First, the number of white blood cells in the peripheral blood was significantly lower and with less transplanted K562 leukemia cells ; Second, liver function damage was alleviated as liver function tests showed that alanine aminotransferase （ ALT）, aspartate aminotransferase （AST） and total bilirubin （TBiL） were significantly reduced, while the albumin （Alb） was notably increased; Third, liver antioxidant ability was improved as the activities of the antioxidant enzymes glutathione peroxidase （GSH-Px） and superoxide dismutase （SOD） were significantly increased, and the contents of GSH and malonaldehyde （MDA） were decreased significantly in the liver; Fourth, the inflammation of the liver was relieved as the level of IL-1β and IL-6, the inflammatory cytokines, were decreased significantly in the liver. Fifth, liver index was incre
出处
《中国中药杂志》
CAS
CSCD
北大核心
2014年第1期121-125,共5页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(81173398)
