摘要
目的探讨DKK1对子宫内膜癌Ishikawa细胞侵袭、迁移能力的影响。方法子宫内膜癌Ishikawa细胞,分别施加外源性重组DKK1,分为对照组(未施加DKK1)、DKK1组(施加终浓度为300 ng/ml DKK1);DKK1小干扰RNA(siRNA)瞬时转染,分为对照组(未转染siRNA)、DKK1 RNA干扰(RNAi)组(转染DKK1 StealthTMSelect siRNA)。应用Transwell实验,计数每个视野中穿过凝胶和微孔滤膜的细胞数,反映细胞的侵袭能力;计数穿过微孔滤膜的细胞数,反映细胞的迁移能力。结果外源性重组DKK1干预后24 h,DKK1组穿过凝胶和微孔滤膜的细胞数为(101.10±6.05),较对照组(135.90±3.98)减少(t=15.20,P<0.05)。外源性DKK1干预后,细胞的侵袭能力减低25.61%。DKK1组穿过微孔滤膜的细胞数为(107.10±5.63),较对照组(142.60±6.95)减少(t=12.56,P<0.05)。外源性DKK1干预后,细胞的迁移能力减低24.89%。DKK1 siRNA转染后24 h,DKK1 RNAi组细胞DKK1mRNA表达水平较对照组降低36.71%。DKK1 RNAi组穿过凝胶和微孔滤膜的细胞数为(150.00±4.83),较对照组(130.40±6.15)增加(t=7.93,P<0.05)。下调DKK1基因表达后,细胞的侵袭能力增强15.03%。DKK1 RNAi组穿过微孔滤膜的细胞数为(154.30±5.64),较对照组(139.70±3.47)升高(t=6.98,P<0.05)。下调DKK1基因表达后,细胞的迁移能力增强10.45%。结论 DKK1能抑制子宫内膜癌Ishikawa细胞的侵袭、迁移能力。DKK1有望成为子宫内膜癌细胞侵袭、迁移的有效靶点。
Objective To investigate the impacts of DKK1 on the invasion and migration ability of endometrial carcino- ma Ishikawa cell lines. Methods Some endometrial carcinoma Ishikawa ceils were divided into control group and DKKI group, the control group was not given any treatment, the DKK1 group was treated with 300 ng/ml DKK1. Other endometrial carcinoma lshikawa cells were also divided into control group and DKK1 RNAi group, the control group was not given any treatment, DKK1 RNAi group was treated with DKK1 StealthTM Select siRNA. By using Transwell test, the cell invasion ability were evaluated by counting cell population which crossed gelatin and micropore in every visual field, the cell migration ability were evaluated by counting cell population which crossed mieropore. Results After 24 h of exogenous recombinant DKK1 intervention, the number of cells which crossed gelatin and micropore in DKK1 group （ 101.10 ± 6. 05 ） were significantly less than those in control group （ 135.90 ± 3.98 ） （ t = 15.20, P 〈 0. 05 ）, the cell invasion ability had dropped by 25.61%. The number of ceils which crossed micropore in DKK1 group （ 107. 10 ± 5.63） were significantly less than those in control group （ 142. 60 ± 6.95 ） （ t = 12.56, P 〈0. 05）, the cell migration ability had dropped by 24. 89%. After 24 h of DKK1 siRNA transfection, compared with the control group, the DKK1 mRNA expression level in DKK1 RNAi group had dropped by 36. 71%, the number of cells which crossed ge- latin and microporc in DKK1 RNAi group （150. 00 ±4. 83 ） were significantly more than those in control group （130. 40 ± 6. 15 ） （ t = 7.93, P 〈 0. 05 ） . With down - regulation of DKK1 expression, the cell invasion ability had increased by 15.03%. The number of ceils which crossed micropore in DKK1 RNAi group （ 154. 30 ± 5.64 ） were significantly more than those in control group （ 139.70 ±3.47） （t =6. 98, P 〈0. 05） . With down - regulation of DKK1 expression, the cell migration ability had in-
出处
《中国全科医学》
CAS
CSCD
北大核心
2013年第36期4279-4283,共5页
Chinese General Practice
基金
北京市优秀人才培养资助项目(2011D003034000035)
