摘要
目的研究五味子木脂素诱导人脑胶质瘤SHG-44神经球细胞凋亡的作用机制。方法四甲基偶氮唑蓝法分析检测五味子木脂素对SHG-44神经球细胞增殖的影响;Annexin V/PI分析凋亡率;酶联免疫吸附法法检测培养上清中bax、bcl-2和caspase 3蛋白分泌的变化,蛋白质印迹法检测神经球bcl-2蛋白表达变化。结果 50、100、200 mg·L-1五味子木脂素作用24、48和72 h时,均能明显抑制SHG-44神经球细胞的增殖,作用呈剂量依赖性。Annexin V/PI结果显示,五味子木脂素可诱导SHG-44神经球细胞明显凋亡,作用亦呈剂量依赖性。酶联免疫吸附法结果显示,不同浓度五味子木脂素作用后,bcl-2蛋白分泌明显下调使bax/bcl-2比例升高,caspase 3蛋白分泌增加。蛋白质印迹结果显示,SHG-44神经球表达bcl-2蛋白明显下降。结论五味子木脂素通过下调bcl-2的表达和分泌,使抑制凋亡因素减弱而促凋亡因素占优势,使caspase 3活化,进而引起SHG-44神经球细胞发生凋亡。
：OBJECTIVE To explore the mechanism of Schisandra lignans for inducing the apoptosis of human glioma SHG-44 neurospheres. METHODS The effects of Schisandra lignans on the SHG-44 neurospheres proliferation were detected by MTT. The apoptotic rate was analyzed by Annexin-V/PI double staining. The secretion of the bax, bcl-2 and caspase 3 protein was detected by Elisa assay. The expression of bcl-2 protein was examined by Western blot. RESULTS Schisandra lignans could inhibit the prolifera- tion of SHG-44 neurospheres with the concentration of 50,100,200 mg ~ L-1 in a dose-dependent manner. Schisandra lignans could in- duce the apoptosis of SHG-44 neurospheres in a dose-dependent manner. The secretion of bcl-2 was decreased, but ratio of bax/bcl-2 was increased, caspase 3 was increased, the expression of bcl-2 protein was down-regulated. CONCLUSION Schisandra lignans could induce the apoptosis of human glioma SHG-44 neurospheres. Its mechanism is correlated with down-regulation of the expression of bcl-2 and up-regulation of the ratio of bax/bcl-2, and then activated caspase 3.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2014年第2期113-116,共4页
Chinese Pharmaceutical Journal
基金
国家自然科学基金资助课题(81201671)
吉林省教育厅基金资助课题(2011281
2012330)
