摘要
目的 建立保健食品中他达拉非、西地那非和伐地那非3种药物的液相色谱-串联质谱检测方法.方法 样品采用甲醇提取,经超声、离心,Agilent ZORBAX C18色谱柱（50mm×2.1mm,1.8μm）分离,梯度洗脱,在LC-MS/MS多反应监测（MRM）模式下进行定性及定量分析.结果 3种药物的检出限：西地那非为：1.0mg·kg-1,他达拉非和伐地那非为：0.2mg·kg-1,在191.7～317.8mg·kg-1范围内,3个添加水平的平均回收率为105.4%～113.4%,相对标准偏差（RSD,n=3）为2.1%～5.7%.结论 该方法可快速、准确地对保健食品中非法添加的西地那非、他达拉非和伐地那非进行定性和定量测定.
OBJECTIVE To establish a method for determination of 3 kinds of drugs of sildenafil, tadalafil and vardenalil in health food. METHODS Sample was extracted with methanol, then handled by ultrasound and centrifuge. The chromatographic separation was using Agilent ZORBAX C18 column （50mm × 2. 1mm, 1.8μm） for gradient elution, then the qualitative and quantitative analysis was carried out with MS/MS （MRM）. RESULTS The detection limit of sildenafil was 1.0mg · kg-1 ,the detection limits of tadalafil and vardenafil all were 0. 2mg ·kg-1. In the range of 191.7 -317.8mg · kg-1 in three levels,the average recoveries were 105.4% - 113.4% ,the relative standard deviations （RSDs） were 2. 1% - 5.7%. CONCLUSION The method is rapid and accurate for the qualitative and quantitative analysis of sildenafil, Tadalafil and Vardenafil in health food.
出处
《海峡药学》
2013年第12期123-125,共3页
Strait Pharmaceutical Journal
