摘要
目的：采用HPLC法测定辽东槐木中槐木皂苷X的含量。方法：色谱柱为Kromasil C18拄（250mm ×4．6mm，5μm）；流动相为乙腈-0．05％磷酸溶液（35：65）；检测波长203nnq；柱温30℃；流速0．8ml／min；进样量：10μl结果：槐木皂苷X在0．60—4．80μg与峰面积具有良好的线性关系（r=0．9999），平均回收率98．14％，RSD为1．13％。结论：该方法灵敏、准确、重复性好，可用于辽东橡木的质量控制。
Objective： To establish a method for determination of Araloside X in root bark of A ralia. Methods： HPLC was performed on a Kromasile Cls column（250 mm x 4.6 mm,5 μm） at 30 ℃.The composition of mobile phase was acetonitrile - 0.05% phosphoric acid（35：65）, the flow rate was 0.8 ml/min and the sample size was 10 μl. Results： The calibration curve showed good linearity over the range of 0.60 - 4.80 μg （r = 0.9999）. The average recovery was 98.14% with RSD of 1.13%. Conclusion： This method is sensitive accurate, reproducible and it can be used for the quality control of root bark of A ralia.
出处
《泸州医学院学报》
2013年第6期585-587,共3页
Journal of Luzhou Medical College
基金
泸州市科技局项目(NO.泸市科[2011]108号)
