摘要
目的：预测小鼠转铁蛋白受体（transferrin receptor, TfR）的B细胞表位，为构建以TfR抗体为脑转运载体的疫苗提供基础。方法：以小鼠转铁蛋白受体基因序列为基础，按Chou-Fasman和Gamier-Robson方法预测其编码蛋白的二级结构，按Kyte-Doolittle方法预测其亲水性，Emini方法预测其表面可能性以及按Jameson-Wolf方法预测其抗原指数。结果：Chou-Fasman和Gamier-Robson方法发现90 - 110, 120 - 145, 160 - 180, 240 - 250, 265 - 275, 290 - 304, 336 - 350, 375 - 383, 426 - 435, 504 - 517, 590 - 602, 613 - 630, 710 - 718这些区域可能为α螺旋的中心区域。用Kyte-Doolittle、 Emini、 Jameson-Wolf方法分别对小鼠TfR的B细胞表位进行预测，结果显示其共有区域为30 - 50, 100 - 115, 145 - 157, 310 - 320, 355 - 360, 440 - 445, 510 - 535, 655 - 660, 690 - 695, 705 - 710。根据抗原表位设计原则筛选出5段符合要求的抗原表位氨基酸序列：DGDNSH, AETEETDKS，NTYTP，MDKNKF，KHPVDGKSLYRDSNWISKV，它们可能为小鼠转铁蛋白受体的B细胞优势表位区域。结论：该结果有助于确定小鼠转铁蛋白受体的B细胞表位以及构建药物-载体复合物，发挥其脑药物转运载体的功能。
Objective： To predict the B-cell epitope of transferrin receptor, for providing a foundation of constructing a vaccine carried by transferrin receptor antibody through the blood-brain barrier. Methods： The secondary structure and hydrophilicity plot were extrapolated using Chou-Fasman, Gamier-Robson and Kyte-Doolittle methods based on the genetic sequence of transferrin receptor. Surface probability plot and antigenic index of transferrin receptor were predicted using the Emini and Jameson-Wolf methods respectively. Results： Centers of α-helix were found in the regions of 90 - 110, 120 - 145, 160 - 180, 240 - 250, 265 - 275, 290 - 304, 336 - 350, 375 - 383, 426 - 435, 504 - 517, 590 - 602, 613 - 630, 710 - 718. B-cell epitope of transferrinreceptor were possibly localized in the regions of 30 - 50, 100 - 115, 145 - 157, 310 - 320, 355 - 360, 440 - 445, 510 - 535, 655 - 660, 690 - 695, 705 - 710. Based on the design principle of antigen epitopes, five sections of amino acid sequence were screaned out which accorded with the requirements： DGDNSH, AETEETDKS, NTYTP, MDKNKF, KHPVDGKSLYRDSNWISKV.These sections may be the potential B - cell epitope region of mouse transferrin receptor. Conclusion： These results are helpful for identifying the dominant B-cell epitope and constructing the drug-carrier compound as a brain drug carrier.
出处
《泸州医学院学报》
2013年第6期580-584,共5页
Journal of Luzhou Medical College
基金
四川省教育厅重点项目(2006A055)
