摘要
目的研究靶向生存素siRNA对喉鳞癌动物模型的抑制作用,探讨其可能的抑瘤机制。方法建立人喉鳞癌裸鼠移植瘤模型,将33只裸鼠随机分为3组,对照组瘤体内注射PBS液,空载体组瘤体内注射空慢病毒载体(Ad-control),治疗组应用生存素siRNA(Ad-survivin)进行瘤内注射治疗,观察各组肿瘤生长情况,光镜及透射电镜观察肿瘤超微结构变化,TUNEL法检测细胞凋亡,免疫组化法检测Bcl-2蛋白的表达情况。结果治疗组体内注射生存素siRNA(100 ng.mL-1.d-1),裸鼠肿瘤生长受到明显抑制,与对照组及空载体组相比,差异有统计学意义(P<0.05)。光镜、电镜下观察到治疗组肿瘤组织发生坏死和凋亡改变,Tunel证实治疗组肿瘤细胞凋亡数目明显增多,与对照组及空慢病毒组相比,差异有统计学意义(P<0.05),免疫组化示治疗组Bcl-2表达下降。结论生存素siRNA抑制喉鳞癌裸鼠生长,其作用机制可能与抑制Bcl-2表达诱导肿瘤细胞凋亡相关。
Objective To investigate the therapeutic effect of survivin siRNA,related arrestant-inducing on human laryngeal squamous carcinoma in nude mice,and to explore the possible mechanism.Methods Nude mouse model bearing laryngocarcinoma was established by using human laryngeal squamous carcinoma cell line （Hep-2）.Thirty-three mice were randomly divided into three groups：the control group intratumoral injection of PBS,empty vector group intratumoral injection of empty lentiviral vector （Ad-control）,and the treatment group was given survivin siRNA.Tumor growth and the tumor microscopic structure changes were observed with light microscopy and transmission electron microscopy.TUNEL was used to detect the apoptosis cells in control group and treatment group.Immunohistochemical technique was used to examine the expression of Bcl-2 protein.Results In vivo,tumor growth was significantly inhibited in treatment group compared to the control group and empty vector group,the difference was statistically significant （P ＜ 0.05）.The necrosis and apoptosis of the tumor cells were found under light and electron microscope.The Tunnel showed cell apoptosis was obvious in treatment group,and Bcl-2 expression was obviously inhibited in immunohistochemistry.Conclusion Survivin siRNA can significantly inhibit the growth of laryngeal squamous carcinoma Hep-2 cell line in vitro,and the growth of laryngocarcinoma in nude mice in vivo,and the inhibitory effects are resulted from the apoptosis-inducing effect of Bcl-2.
出处
《哈尔滨医科大学学报》
CAS
北大核心
2013年第3期212-215,共4页
Journal of Harbin Medical University
