摘要
目的评价丙泊酚后处理对脂多糖（LPs）诱导大鼠脑组织小胶质细胞炎性反应的影响。方法原代培养sD大鼠全脑小胶质细胞，以1×10^5个／ml密度接种于24孔培养板（1ml／孔），100个培养孔，采用随机数字表法，将其分为5组（n=20），正常对照组（c组）常规培养；L组加入1μg／ml LPS孵育24h；P25组、P50组和P100组于1μg／ml LPS孵育24h时加入丙泊酚，终浓度分别为25、50、100μmol／L。于丙泊酚孵育1h时收集细胞，采用RT-PCR法检测诱导型-氧化氮食酶（iNOS）mRNA、环氧化酶-2（COX-2）mRNA、肿瘤坏死因子-α（TNF-α）mRNA和自细胞介素-1β mRNA的表达水平；收集上清液，采用Griess法测定-氧化氮（NO）浓度，采用酶联免疫吸附法测定前列腺素E2（PGE2）、TNF-α和IL-1β的浓度。结果与C组比较，L组iNOSmRNA、COX-2mRNA、TNF-α mRNA和IL-1βmRNA的表达及上清液NO、PGE2、TNF-α和IL-1β的浓度升高（P〈0．01）；与L组比较，P50组和P100组iNOSmRNA、COX-2mRNA、TNF-αmRNA和IL-1βmRNA的表达及上清液NO、PGE2、TNF-α和IL-1β的浓度降低（P〈0．05或0．01），而P2，组上述指标差异无统计学意义（P〉0．05）。结论50、100μmol／L丙泊酚后处理可抑制LPS诱导大鼠脑组织小胶质细胞炎性反应。
Objective To evaluate the effects of postconditioning with propofol on lipopolysaccharide （LPS）-induced inflammatory responses of microglial cells in rat brain tissues.Methods The primary cultured mi- croglial cells in brain tissues of Sprague-Dawley rats were seeded in 24 multi-well plates at a density of 1 × 10^5 cells/ml, and the microgtial cells of 100 wells were randomly divided into 5 groups （ n = 20 each） using a random number table： control group （group C）, LPS group （group L）, and propofol 25, 50 and 100 μmol/L groups （P25, P50, P100 groups）. The cells were cultured routinely in group C. LPS 1 μg/ml was added and the cells were incu- bated for 24 h in group L. In P25 , P50 , and P100 groups, when the cells were incubated for 24 h with LPS 1μg/ml, propofol with the final concentrations of 25, 50 and 100 μmol/L was added, respectively. The cells were collected at 1 h of incubation with propofol for determination of the expression of inducible nitric oxide synthase （iNOS） mR- NA, eyelooxygenase-2 （COX-2） mRNA, tumor necrosis factor-α （TNF-α） mRNA and intedeukin-1β （IL-1β） mR- NA （by RT-PCR）. The supernatant was separated for determination of the concentrations of nitric oxide （NO） （by Griess method） and prostaglandin E2 （PGE2）, TNF-α and IL-1β （by ELISA）. Results Compared with group C, the expression of iNOS mRNA, COX-2 mRNA, TNF-α mRNA and IL-1β mRNA was significantly up-regulated and the concentrations of NO, PGE2, TNF-α and IL-1β in the supernatant were increased in group L （P 〈 0.01 ）. Compared with group L, the expression of iNOS mRNA, COX-2 mRNA, TNF-α mRNA and IL-1β mRNA was sig-nificantly down-regulated and the concentrations of NO, PGE2, TNF-α and IL-1β in the supernatant were decreased in P50 and P100 groups （P 〈 0.05 or 0.01）, while no significant change in the indexes mentioned above was found in P25 group （ P 〉 0.05）. Conclusion Postconditioning with propofol 50 and 100μmol/L can inhibit LPS-induced
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2013年第11期1383-1385,共3页
Chinese Journal of Anesthesiology
基金
国家自然科学基金青年基金（30901393）
