摘要
目的观察宫颈癌新辅助化疗相关的差异miRNA表达谱,明确与宫颈癌新辅助化疗相关的miRNA表达分子机制。方法从宫颈癌患者新辅助化疗前后的肿瘤组织标本中筛选出3对标本(均为宫颈癌Ⅱb期高分化鳞状上皮细胞癌),利用Agilent microRNA芯片检测法,初步筛选与新辅助化疗相关的miRNA表达谱。结果初步筛选出56个差异表达的miRNA,其中29个miRNA在新辅助化疗后表达上调,占总数的52%;27个miRNA表达下调,占总数的48%。结合文献筛选出5个宫颈癌相关的miRNA。结论宫颈癌新辅助化疗与5个miRNA的差异性表达具有相关性。
Objective It is to observe the differential expression profile correlated with neoadjuvant chemotherapy for cervi-cal cancer, thus to clear and definite the molecular mechanism of these expressions. Methods Three pairs of samples ( all were cervical cancer that were stage Ⅱb well-differentiated squamous cell carcinoma) were selected from tumor tissue in the patients with cervical cancer before and after neoadjuvant chemotherapy. The differential expression profile correlated with neoadjuvant chemotherapy was found out after initial screening with Agilent microRNA chip detection. Results 56 miRNA of differential ex-pression were found out after initial screening, in which 29 miRNA accounting for 52% were over-active and 27 miRNA ac-counting for 48% were under-active after neoadjuvant chemotherapy. Finally 5 miRNA that were related with cervical cancer were screened combined with literature. Conclusion Neoadjuvant chemotherapy is related with the differential expression of 5 miRNA.
出处
《现代中西医结合杂志》
CAS
2014年第2期122-124,139,共4页
Modern Journal of Integrated Traditional Chinese and Western Medicine
基金
陕西省社发攻关资助项目(2010K01-156)
关键词
宫颈癌
新辅助化疗
MIRNA
cervical cancer
neoadjuvant chemotherapy
miRNA