摘要
用研发的克仑特罗化学发光酶联免疫(CLEIA)试剂盒与克仑特罗酶联免疫(ELISA)试剂盒进行检测效果比对,结果显示,CLEIA试剂盒检测时间为33 min,而ELISA试剂盒则需要50 min,CLEIA试剂盒方法要比ELISA试剂盒方法更节省时间;CLEIA试剂盒IC50为40.0 ng·L-1,对猪尿和猪肉的最低检测限分别为20.1 ng·L-1和90.7 ng·kg-1,而ELISA试剂盒IC50则为125.2 ng·L-1,对猪尿和猪肉的最低检测限分别为23.5 ng·L-1和99.3 ng·kg-1,CLEIA试剂盒方法的灵敏度要高于ELISA试剂盒方法的灵敏度。综合分析得知:CLEIA法比ELISA法灵敏度更高、反应时间更短。
A comparative analysis with clenbuterol chemiluminescence enzyme-linked immunoassay （ CLEIA） kit and clenbuterol enzyme-linked immunosorbent assay （ ELISA） kit was used to determined the clenbuterol contents in sam-ples of porcine urine and pork .The results showed that the testing time of the CLEIA kit was 33 min, and that of the ELISA kit was about 50 min, so the CLEIA kit cost less time than ELISA kit .The IC50 of the CLEIA kit was 40.0 ng · L-1 , and the limit of detection for urine and pork were 20.1 ng· L-1 and 90.7 ng· kg-1 , respectively , and the ho-mologous parameters of ELISA kit were 125.2 ng· L-1 , 23.5 ng· L-1 and 99.3 ng· kg -1 , so the sensibility of CLEIA method was better than ELISA method .In summary , the CLEIA method was more sensitive and cost less time than ELISA method .
出处
《浙江农业学报》
CSCD
北大核心
2013年第6期1360-1363,共4页
Acta Agriculturae Zhejiangensis
基金
首都科技条件平台项目化学发光免疫分析仪关键模块研发与产业应用培育课题(Z121100008812002)
关键词
克仑特罗
化学发光酶联免疫
酶联免疫
clenbuterol
chemiluminescence enzyme-linked immunoassay
enzyme-linked immunosorbent assay
