摘要
目的 制备以B群脑膜炎球菌外膜蛋白（outer membrane protein,OMP）为载体的AC群脑膜炎球菌多糖（polysaccharide,PS）结合疫苗（AC-OMP）,并初步研究该制备工艺的稳定性.方法 将以溴化氰活化的A、C群PS分别与B群OMP偶联制成AC-OMP.按《中华人民共和国药典》2010年版三部的要求对制备的AC-OMP进行检定.以制备的AC-OMP免疫小鼠,用ELISA法检测小鼠血清抗体阳转率.结果 制备的AC-OMP的各项指标均达到质控标准.AC-OMP具有良好的免疫原性,AC-OMP免疫小鼠的血清抗PS抗体阳转率为100％.结论 该工艺适用于AC-OMP制备.
Objective To prepare groups A and C meningococcal polysaccharide （PS） conjugate vaccine with outer membrane protein （OMP） of group B meningococcus as a carrier （AC-OMP）,and preliminarily study on stability of the preparation process for AC-OMP.Methods Groups A and C meningococcal polysaccharides activated by cyanogen bromide were coupled with OMP of group B meningococcus respectively to prepare monovalent A-OMP and C-OMP,then AC-OMP was prepared by mixing monovalent A-OMP and C-OMP.The prepared AC-OMP was detected according to the requirements of Chinese Pharmacopoeia 2010 （Volume Ⅲ）.Mice were immunized with the prepared AC-OMP,and seroconversion rates of anti-PS in immunized mice were detected by ELISA.Results Each index of the prepared AC-OMP achieved quality control standard.AC-OMP had a good immunogenicity,and seroconversion rates of anti-PS were 100％ in immunized mice.Conclusion The preparation process is suitable for AC-OMP.
出处
《国际生物制品学杂志》
CAS
2013年第6期281-283,共3页
International Journal of Biologicals
关键词
奈瑟球菌
脑膜炎
疫苗
结合
多糖类
细菌
细菌外膜蛋白类
Neisseria meningitidis
Vaccines, conjugate
Polysaccharides, bacterial
Bacterial outer membrane proteins
