摘要
目的了解终末消毒前后物体表面微生物的变化情况,为医院感染防控提供依据。方法对神经外科ICU终末消毒前、后进行环境卫生学采样,样本进行细菌培养与鉴定,比较终末消毒前、后物体表面细菌数量及种类的变化。结果神经外科ICU终末消毒前,27.45%的物表菌落数达到物表消毒效果标准(≤5CFU/cm2),终末消毒后88.24%的物表菌落数达标。消毒前后物表微生物均以凝固酶阴性葡萄球菌(89.22%/34.41%)、革兰阳性杆菌(37.25%/39.22%)、微球菌(29.41%/12.75%)、霉菌(10.78%/18.63%)为主;消毒前检出最多的医院感染病原菌是鲍曼不动杆菌,符合神经外科医院感染特点;消毒前检出的临床常见条件致病菌在消毒后绝大多数被杀灭,同时也检出少量其他的条件致病菌,主要分布于潮湿、平时容易忽视、难清洁消毒及接触频繁的物表。结论终末消毒效果较为明显,应定期对病房进行终末消毒,保证病房环境的清洁卫生。
Objective To understand neurosurgery ICU in the hospital before and after terminal disinfection the bacteria carriage status in ward of the surface and provide the basis for the prevention and control of hospital infection. Methods The surface in neu- rosurgery ICU were sampling for environmental hygiene before and after terminal disinfection. Samples were cultured and identi- fied, and then surface changes in the number and type of bacteria before and after terminal disinfection were compared. Results 27.45% of the number of surface colonies reached the level of disinfection efficacy standards in the surface（≤5 CFU/cm^2） before terminal disinfection, the number of 88.24% of surface colonies was less than or equal 5 CFU/cm^2 after terminal disinfection. The majority of microorganism was coagulase-negative Staphylococci （ 89.22 % /34.41 % ）, Gram positive bacilli（ 37.25 % /39.22 % ）, mi- crococcus（29. 41%/12. 75%） ,fungi（10.78%/18.63%） before and after disinfection from surface microbes. The major pathogens of hospital infection detected before disinfection were Acinetobacter baumannii, which was in line with the characteristic of depart- ment of neurosurgery. The majority of special conditions pathogens that were presence before disinfection were killed after terminal disinfection ,however, the new other conditions pathogens were also detected, which mainly survived in the surface that were humid, easily ignored, cleaned difficultly, contacted frequently. Conclusion The effects of terminal disinfection are very obvious, and this method should be carried out regularly in the ward to keep the environment clear.
出处
《国际检验医学杂志》
CAS
2013年第24期3274-3276,共3页
International Journal of Laboratory Medicine
基金
国家自然科学基金资助项目(71373280)
关键词
神经外科
重症监护病房
终末消毒
细菌
neurosurgery
intensive care units
terminal disinfection
bacteria
