摘要
目的探讨髓源抑制细胞（MDSCs）在胃肠道恶性肿瘤患者外周血的表达及临床意义。方法收集20名健康志愿者（年龄28～50岁，平均36岁）和59名新诊断的胃肠道恶性肿瘤患者（年龄27。80岁，平均59岁）的外周血，用荧光标记的抗人Linl、HLADR、CD33及CDllb单克隆抗体标记肿瘤患者和健康人的外周血细胞．应用流式细胞分析术检测MDSCs（Lin-/Le HLA DR-CD33＋ CD11b＋）细胞的表达。结果胃肠道恶性肿瘤患者的MDSCs百分比为（3．99±1．241％，明显高于健康人群（1．81±0．74，P〈O．01）；伴肿瘤转移者（5．34±1．54）%和非转移肿瘤患者（3．71±1．46）％比较，有统计学差异（P〈0．051：胃癌患者MDSCs（3．57±1．59）％和肠癌（4．31±1．71）％相比，无统计学意义（P〉O．051；所有肿瘤患者中，同时患有高血压、糖尿病者19例。MDSCs平均（3．62±1．16）％，和一般肿瘤病人比较，无差异（Jp〉0．05）。结论MDSCs在胃肠道恶性肿瘤中表达增高，肿瘤继发转移者增加更明显，可用于判断病情和预后；但在不同组织来源肿瘤中的表达不具有特异性，高血压、糖尿病等基础疾病对MDSCs在影响肿瘤发生发展方面无明显影响。
Objective To explore the expression and clinical significance of myeloid-derived suppressor cells （MDSCs） in pe- ripheral blood in patients with gastrointestinal cancer. Methods The peripheral blood samples were Collected from 20 healthy vol- unteers （aged 28 to 50 years, mean 36 years） and 59 newly diagnosed patients （aged 27 to 80 years, mean 59 years） with gastroin- testinal cancer. The peripheral blood cells from tumor patients and healthy persons were labeled with fluorescent labeled anti-hu- man Linl, HLA DR, CD33 and CD1 lb monoclonal antibody. The MDSCs（Lin-/Lo HLA DR-CD33＋ CD11b＋） were detected with flow cytometry. Results The percentage （3.99±1.24%） of MDSCs in patients with gastrointestinal cancer was significantly higher than the healthy persons （1.81±0.74%, P〈0.01）; Significant difference existed between metastatic tumors （5.34±1.54%） and non- metastatic tumors （3.71±1.46%）.There was no statistical significance（P〉0.05） in MDSCs between patients with gastric cancer （3.57± 1.59%） and patients with colon cancer （4.31±1.71%）. There were 19 cases among all cancer patients also suffering from high blood pressure and diabetes. The average level of MDSCs was （3.62±1.16%）, and there was no difference when compared with patients of general cancer （P〉0.05）. Conclusion MDSCs increases in gastrointestinal malignant tumor, and more obviously in metastatic tu- mors, which can be used to determine the severity and prognosis of tumors. But it is not specific in tumors with different tissue source. High blood pressure, diabetes and other basic diseases influence tumors＂ development slightly in MDSCs.
出处
《实验与检验医学》
CAS
2013年第6期554-555,558,共3页
Experimental and Laboratory Medicine
