摘要
目的通过检测原发性骨质疏松症患者骨组织基因表达谱的差异,探讨原发性骨质疏松症肾阴虚证相关基因的信息学特征。方法随机选择骨质疏松症患者,中医辨证分型为肾阴虚证组3例、肾阳虚证组3例、无肾虚证组3例,并选择正常骨密度人群3例设为正常对照组。用人全基因组表达谱芯片检测4组人群骨组织基因表达谱,筛选共同的差异表达基因,并对这些差异表达基因进行基因通路等相关功能分析。结果肾阴虚证组与正常对照组、肾阳虚证组、无肾虚证组的差异表达基因分别为2378、4026、4071条,肾阴虚证组与其他3组比较共同的差异表达基因有344条,这些差异基因参与免疫调节、矿物质吸收、激素合成、组氨酸代谢等11条信号通路。结论原发性骨质疏松症肾阴虚证的相关基因主要与免疫调节、激素合成、组氨酸代谢、矿物质吸收等信号通路相关。
Objective To investigate the characteristics of genes expression profiles of kidney yin deficiency in primary osteoporosis by analyzing the difference of gene expression profiles in the bone tissues in patients with primary osteoporosis. Methods Osteoporotic patients were randomly selected. All the patients were divided into kidney yin deficiency group, kidney yang deficiency group, and non-kidney deficiency group according the syndrome differentiation of traditional Chinese medicine. Each group had 3 patients. Another 3 people with normal bone mineral density （BMD） were selected as normal control. Gene expression profiles of the bone tissues in all 4 groups were detected using the human genome-wide expression profile chip. The differentiated expression genes were screened, and the analysis of the related functions of these genes including the pathways was conducted. Results Compared with that in kidney yang deficiency group, non-kidney deficiency group, and normal control group, the number of differentiated expression genes in kidney yin deficiency group was 2378, 4026, and 4071, respectively. Compared with that in these 3 groups, the number of common differentiated expression gene in kidney yin deficiency group was 344. These genes were involved in 11 pathways, including immunoregulation, mineral absorption, hormone synthesis, and histidine metabolism. Conclusion Related genes of kidney yin deficiency syndromes in primary osteoporosis are mainly related to the signal pathways such as immunoregulation, hormone synthesis, histidine metabolism and mineral absorption.
出处
《中国骨质疏松杂志》
CAS
CSCD
北大核心
2013年第12期1215-1218,1253,共5页
Chinese Journal of Osteoporosis
基金
国家自然科学基金(81173280)
福建省科技厅省属公益类科研院所基本科研专项(2011R1038-5)
福建省卫生厅创新课题(2011-CX-30)
福建省卫生厅青年基金项目(2010-1-31)
关键词
原发性骨质疏松症
肾阴虚证
基因表达谱
Primary osteoporosis
Kidney yin deficiency syndrome
Gene expression profile
