摘要
目的:探讨联合检测对提高HCV RNA在慢性丙型肝炎患者中检出率的临床意义。方法:利用逆转录聚合醇链反应(RT-PCR)技术对583例ALT升高持续1年以上的患者进行同步检测血浆和外用血单个核细胞(PBMC)中的HCV RNA。结果:血浆中HCV RNA总检出率为19.2%。PBMC中HCV RNA总检出率为24.5%.HCV RNA在PBMC中的检出率明显高于血浆中的检出率.结论:两种方法同时应用能够提高HCV RNA在慢性丙型肝炎患者中的检出率。
Aim: To increase the HCV RNA detectable rate of the chronic Hepatitis C patients. Method: To detect synchronously the HCV RNA in Plasma and PBMC of Hepatitis C patients whose ALT keeping arising continually for more than one year by using the TR - PCR. Results the final detectable rate of plasma was 19. 2% and the final detectable rate of HCV RNA in PBMC was 24. 5% and the test result demonstrated that the detectable trai of HCV RNA in PBMC was higher than that of plasma. Conclusion; to detect synchronously HCV RNA in PBMC could increase the detectable rate of HCV RNA of Hepatitis C patients.
出处
《中西医结合肝病杂志》
CAS
2000年第5期5-6,共2页
Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases
