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胃旁路术对血糖及胰岛细胞的影响 被引量:2

Effect of gastric bypass surgery on fasting blood glucose and pancreatic islet cell in type 2 diabetic rats
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摘要 目的研究胃旁路术(gastricbypasssurgery,GBP)对糖尿病大鼠血糖的控制效果及对胰岛细胞的影响。方法采用链脲佐菌素建立糖尿病SD大鼠模型20只,随机数表法分为糖尿病手术组(DO组)和糖尿病对照组(DC组),另取20只非糖尿病大鼠随机数表法分为正常对照组(NC组)和正常手术组(NO组)。DO组和NO组大鼠行GBP,DC组和NC组大鼠行假手术,分别检测各组大鼠术前、术后72h、1、4和8周空腹血糖水平。术后8周,取胰腺组织行HE染色和免疫荧光,观察组织学变化。结果术前DO组与DC组及NC组与NO组大鼠空腹血糖之间比较差异均无统计学意义(P〉0.05)。DO组大鼠术后空腹血糖进行性下降(P〈0.05)。DC组大鼠术前及术后各时相的血糖差异无统计学意义(P〉0.05);NO组和NC组大鼠组内不同时相及组间相同时相空腹血糖之间比较差异均无统计学意义(P〉0.05)。镜下HE染色:手术组大鼠胰腺内出现新生的小胰岛,胰岛数目增加,这些新生的胰岛体积较小,大部分出现在胰管周围且结构接近正常胰岛;免疫荧光染色显示:胰岛数目增加,胰岛素免疫荧光发现较多孤立的及由两三个胰岛素阳性细胞组成的小胰岛。胰岛素和胰高血糖素免疫荧光双标发现在部分胰岛内存在胰岛素和胰高血糖素双阳性(INS^+/GLU^+)的细胞。结论GBP对2型糖尿病大鼠具有明显的降糖作用,胰岛再生在其中起着重要作用,但对正常大鼠血糖无影响。 Objective To study the effect and mechanism of gastric bypass surgery on type 2 diabetic rats. Methods The models of type 2 diabetic rats were induced by stretozotocin and 20 diabetic rats were randomly divided into 2 groups : diabetes-operation group ( DO group, n = 10) and diabetes-control group ( DC group, n = 10). 20 normal rats were randomly divided into 2 groups: normal-operation( NO group, n = 10)and normalcontrol group( NC group, n = 10). Rats in DO and NO group underwent GBP and rats in DC group and NC group underwent sham operation. Fasting blood glucose(FBG) levels of rats in each group were detected before operation and on 72 h, 1 th week,4th week,8th week after operation. On the 8th week after operation, pancreas tissues were harvested for HE staining and immunofluorescence, histological changes observed. Results The FBG levels of rats were not statistically significant different before operation between DO group and DC group or between NO group and NC group ( P 〉 0. 05 ). After operation, the FBG levels of rats in DO group gradually declined ( P 〈 0. 05 ). FBG levels of rats in DO group were lower after operation than before operation( P 〈 0. 05 ) ; After opera- tion FBG levels of rats were higher in DO group than in NO group and NC group at the same time point ( P 〈 0. 05 ). In DC group, the difference of FBG levels of rats at different time point was not statistically significant( P 〉 0. 05 ). The difference of FBG had no statistically significance between the different time points of the same group or between the same time point of different groups ( P 〉 0. 05 ). HE staining showed that, in DO group, newborn small islets appeared in pancreas which increased the number of islet. The new islets were smaller, mostly around the pancreatic duct and the structure was similar to that of the normal islets. Immunofluorescence staining also showed that the number of islets increased. Insulin immunofluorescence found more isolated small islets com
出处 《中华内分泌外科杂志》 CAS 2013年第6期454-457,共4页 Chinese Journal of Endocrine Surgery
基金 基金项目:浙江省温岭市科技计划项目(2012WLCB0097)
关键词 胃旁路术 2型糖尿病 血糖 胰岛细胞再生 Gastric bypass surgery Type 2 diabetes Fasting blood glucose Regeneration of pancreas islet
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