摘要
采用杂交瘤技术制备出莪术醇的单克隆抗体（Mab）,对莪术醇的酶联免疫吸附分析法（ELISA）进行线性、精密度、重复性、加样回收率等方面的评价,并与气相色谱分析法（GC）进行比较.结果显示,酶联免疫吸附分析法的线性范围为0.16～11.00 μg/ml,重现性好,精密度高,平均加样回收率为102.78％,板间和板内差异均小于10.00％,最低检测限为0.078 μg/ml.用该方法测定广西莪术、温郁金中莪术醇含量,其检测结果与GC方法进行比较,二者的相关系数达到0.972 8.由此可知,莪术醇的ELISA检测方法总体上符合免疫分析的要求.
The curcumol monoclonal antibody prepared by hybridoma technique was used for the determination of curcumol by enzyme-linked immunoassay （ELISA）. The accuracy, repeatability, and recovery of the method was assessed. The method showed good repeatability and high accuray. The average recovery was 102.78% , and the lowest detection lim- it was 0. 078μg/ml. The contents of curcumol in Curcuma kwangsiensi and Curcuma wenyujin detected by ELISA was pos- itively correlated with those by gas chromatography（ GC）, with a correlation coefficient of 0. 972 8. The results suggested that ELISA met the requirements for detection of eurcumol.
出处
《江苏农业学报》
CSCD
北大核心
2013年第6期1468-1471,共4页
Jiangsu Journal of Agricultural Sciences
基金
国家自然科学基金资助项目(30960498)
广西自然科学基金资助项目(2010GXNSFA013252)
