摘要
目的观察姜黄素对脓毒症急性肺损伤大鼠NF-κB相关信号通路和炎症反应的影响,探讨姜黄素对急性肺损伤组织的保护作用及其分子机制。方法 SD大鼠随机分为3组(每组10只):假手术组、模型对照组及姜黄素干预组。盲肠结扎穿刺(CLP)术后12 h后,收集血液及肺组织标本。酶联免疫吸附法(ELISA)检测血清中肿瘤坏死因子-α(TNF-α)、细胞间黏附分子-1(ICAM-1)及巨噬细胞炎症蛋白-2(MIP-2)水平;逆转录多聚酶链反应法(RT-PCR)检测肺组织TNF-α、ICAM-1及MIP-2 mRNA的表达;凝胶电泳迁移率实验(EMSA)法检测肺组织细胞核内核因子-κB(NF-κB)DNA结合活性。结果①与假手术对照组比较,模型组血清TNF-α、ICAM-1及MIP-2水平和肺组织TNF-α、ICAM-1及MIP-2 mRNA的表达均明显增加(P<0.01),模型组肺组织细胞核内NF-κB DNA结合活性明显增加(P<0.01)。②与模型组比较,姜黄素干预组血清TNF-α、ICAM-1及MIP-2水平和肺组织TNF-α、ICAM-1及MIP-2 mRNA的表达均明显降低(P<0.01),姜黄素干预组肺组织细胞核内NF-κB DNA结合活性降低(P<0.01)。结论姜黄素对脓毒症ALI大鼠具有抗炎作用,其机制可能与抑制NF-κB活性从而减少相关炎性基因和蛋白表达有关。
[Objective] To study the molecular mechanism of the effect of curcumin inhibiting the inflammation and NF—κB signaling pathway in septic acute lung injury rats. [Methods] SD rats were randomly divided into 3 groups (n =10): sham group; model group; curcumin treatment group. Rats were killed at 12 h after CLP to collect blood and lung tissue. Serum levels of TNF-α, MIP-2 and ICAM-lwere measured by ELISA, TNF-α, MIP-2 and ICAM-1 mRNA of lung were measured by RT-PCR, the nuclear NF—κB DNA binding activity were measured by EMSA. [Results ] Compared with the sham operation group, levels of serum TNF-α, ICAM-1 and MIP-2 and lung tissue TNF-α, ICAM-1 and MIP-2 mRNA expression increased significantly in model group (P 〈0.01); compared with sham operation group, nuclear NF—κB DNA binding activity of lung tissue increased significantly (P 〈0.01). Compared with model group, levels of serum TNF-α, ICAM-1 and MIP-2 and lung tissue TNF-α, ICAM-1 and MIP-2 mRNA expression decreased significantly in curcumin treatment group(P 〈0.01); Compared with model group, nuclear NF—κB DNA binding activity of lung tissue decreased significantly(P 〈0.01). [ Conclusions ] Effect of curcumin on septic ALI rats may be related to its inhibition of NF—κB signaling pathway to reduce the expression of inflammation-related gene and protein.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2013年第30期19-22,共4页
China Journal of Modern Medicine