摘要
目的观察丙泊酚对内毒素(LPS)诱导人单核细胞(THP-1)表达超氧化物歧化酶(SOD1)的影响及对细胞因子释放的影响。方法将体外培养的人单核细胞THP1细胞分为四组:正常对照组(C组)、LPS刺激组(L组)、丙泊酚处理组(P组)和丙泊酚预处理合并LPS刺激组(P+L组),并用Western blot法检测各组内SOD1含量的变化。采用ELISA法检测四组不同处理因素对THP1细胞分泌IL6、IL8、肿瘤坏死因子α(TNF-α)的影响。结果在LPS刺激12 h后收集细胞,与对照组比较L组中SOD1的表达量明显降低,IL6、IL8、肿瘤坏死因子α(TNF-α)均明显增高(P<0.01);L+P组中SOD1的表达量明显高于L组,IL6、IL8、TNF-α均明显降低(P<0.01)。结论体外THP1细胞培养下,丙泊酚可以明显抑制LPS刺激THP1细胞IL6、IL8、TNF-α的大量释放和逆转LPS刺激下对THP1细胞内SOD1表达的抑制。
Objective To explore the effect of propofol on secreting cytokines and SOD1 expression from human mononuclear macrophage cell induced by lipopolysaccharide （LPS）. Methods THP-1 cultured in vitro wererandomly assigned into four groups： control group, LPS group, propofol group, LPS and pretreatment with propofol group. Western blot was applied to detect the expression of SOD 1, and ELISA was used to detect cytokins secreted by THP1 cells stimulated with LPS. Results 12 h after stimulated by LPS, compared with the control group, the expres- sion of SOD1 in LPS group decreased significantly, but cytokins （IL6, IL8, TNF-~t） were higher （P〈0.01）. Compared with LPS gruop, the expression of SOD1 in LPS and pretreatment with propofol group was higher, but cytokins （IL6, IL8, TNF-a） decreased significantly （P〈0.01）. Conclusion Propofol can increase the expression of SOD1, but de- crease cytokins, and it may have some anti-peroxidation effects.
出处
《海南医学》
CAS
2013年第24期3592-3594,共3页
Hainan Medical Journal
