摘要
目的 :研究脑蛋白水解物的组份测定及小鼠缺氧和记忆障碍的疗效。方法 :用氨基酸分析仪、高效液相色谱仪、SDS凝胶电泳和琥珀酸脱氢酶法测定脑蛋白水解物的组成与活性。用常压耐缺氧实验和记忆获得障碍法测定脑蛋白水解物的疗效。结果 :脑蛋白水解物的氨基酸与小分子肽的比例分别为 80 %与 2 0 %左右。脑蛋白水解物能增强脑细胞琥珀酸脱氢酶活性并对动物缺氧及记忆再现障碍有明显的改善作用。结论 :脑蛋白水解物富含人脑必须氨基酸及小分子肽 。
Aim To study physicochemical property and therapeutic effectiveness of cerebroprotein hydrolysate.Methods To determine the composition and activation of cerebroproptein hydrolysate by aminoacid analyzer,HPLC,SDS-PAG and succinate denydrogenase,to determine the therapeutic effectiveness of cerebrprotein hydrolysate by ordinary pressure oxytolerant experiment and paramnesia experiment.Results The proportion of amino acid and small molecular peptid in cerebroprotein hydrolysate has effect on inproving paramnesia and increasing the activation of succinate dehydrogenase of brain cells in mice.Conclusion Cerebroprotein hydrolysate contains abundantly essential amino acid and small molecular peptid to human brain wich can inprove functional activation of human brain cells.
出处
《解放军药学学报》
CAS
2000年第6期294-296,共3页
Pharmaceutical Journal of Chinese People's Liberation Army
关键词
脑蛋白水解物
记忆错误
缺氧
东莨菪碱
Cerebroprotein hydrolysate
Aminoacid
Peptide
Oxytolerant
Paramnesia
