摘要
为了实现微小尺度目标的光谱成像分析,提出了一种基于像面干涉技术的高光谱显微成像方法。研究了由显微物镜、准直镜、横向剪切分束器、成像镜和探测器等组成的干涉成像光学系统。采用可调式实体横向剪切分束器,并通过旋转横向剪切分束器的方法获得目标的干涉信息。分析了该方法的成像原理、系统放大率、空间分辨率、光谱分辨率以及景深等。对蚕豆叶表皮切片进行了显微光谱成像实验,获得了可见光波段的光谱复原图像。实验结果表明,该方法具有光通量大、光谱分辨率高的优点,为显微光谱成像提供了一种新方法。
In order to obtain the spectral images of objects with small scale, a method of hyperspectral microscopic imaging based on the image plane interferometry is proposed. The interferometric imaging system with microscope objective, collimating lens, lateral shearing beam splitter, imaging lens and detector is researched. An adjustable solid structure of the lateral shearing beam splitter is adopted and rotated to achieve the interferogram of the object. The microscopic imaging principle, magnification, spatial resolution, spectral resolution and depth of field are analyzed. An experimental prototype is constructed to carry out experiments on the epidermis of broad bean leaves specimen, and the spectral images in visible band are recovered. Experimental results validate the good performance of the system with high throughput and high spectral resolution, and show that a new method for the microscopic spectral imaging is provided.
出处
《光学学报》
EI
CAS
CSCD
北大核心
2013年第12期105-112,共8页
Acta Optica Sinica
基金
国家自然科学基金(61205016
U1231112)
教育部高等学校博士学科点专项科研基金(20123219120021)
中国科学院天文光学技术重点实验室开放课题
关键词
成像系统
光谱学
高光谱显微成像
二次成像
横向剪切分束器
imaging systems
spectrocopy
hyperspectral microscopic imaging
re-imaging
lateral shearing beam splitter
