摘要
目的 :探讨肿瘤坏死因子α (tumornecrosisfactorα ,TNF α)对晶状体上皮细胞在细胞外基质上的粘附移行作用 ,以及在此过程中整合素分子的表达情况。方法 :借助细胞粘附实验和移行实验研究TNF α对体外培养的牛晶状体上皮细胞在细胞外基质上的粘附和移行 ,以及整合素抗体封闭后对细胞的影响。TNF α对细胞表面整合素的表达调节经免疫荧光染色后由流式细胞仪检测。结果 :TNF α可以明显促进晶状体上皮细胞在Ⅳ型胶原、层粘蛋白、纤维连接蛋白上的粘附移行。抗体封闭实验表明整合素 β1、α2、α3、α5亚基参与细胞的粘附 ,在细胞移行中发挥主要作用的是α亚基而非 β亚基。流式细胞仪检测表明TNF α可上调晶状体上皮细胞中整合素的表达。结论 :胶原、层粘蛋白、纤维连接蛋白是晶状体囊的主要成分 ,肿瘤坏死因子α促进晶状体上皮细胞与晶状体囊膜成分的粘附移行 ,该作用的发挥至少部分通过TNF
Objective:The pathogenesis of posterior capsule opacification has correlation with migration, proliferation and differentiation of residual lens epithelial cells (LECs) after cataract surgery The aim of this study was to explore the effects of tumor necrosis factor α (TNF α) on LECs' adhesion and migration on extracellular matrix, and the expression of integrins during the course Methods:The cell adhesion and migration assays were used to assess the effects of TNF α on LECs' adhesion and migration on extracellular matrix, and the influence of LECs' incubation with five different monoclonal antibodies specific for integrin alpha subunits 2, 3, 5 and beta subunits 1, 2 The regulation of integrins expression by TNF α was studied by fluorescence activated cell sorting (FACS) technique using indirect immunofluorescent staining Results:TNF α could significantly promote LECs' adhesion and migration on type Ⅳ collagen, laminin and fibronectin The most effective concentration of TNF α was 10 U/ml According to the results of antibody blocking, β1, α2, α3, α5 subunits have involved in cell adhesion and α subunits play a role in cell migration The integrins expression on LECs was obviously up regulated by TNF α Conclusion: As collagen, laminin, fibronectin are the main components of lens capsule, TNF α could enhance the interactions between lens epithelial cells and lens capsule This is achieved partly through the up regulation of integrins expression on lens epithelial cells by TNF α
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2000年第12期652-655,共4页
Chinese Journal of Immunology
关键词
肿瘤坏死因子Α
晶状体上皮细胞
粘附
白内障
Tumor necrosis factor α Lens epithelial cell Adhesion Migration Extracellualr matrix Integrin
