摘要
目的 :研究登革病毒E基因免疫的可行性。方法 :用脂质体转染法将构建的E基因重组真核表达质粒pcDNA3 E导入小鼠成纤维细胞NIH3T3细胞 ,SDS PAGE和蛋白质印迹试验检测E基因的体外表达。然后将该重组真核表达质粒经肌肉注射免疫BALB/c小鼠 ,检测其诱发特异性免疫应答水平。结果 :重组真核表达质粒pcDNA3 E在小鼠体内诱发一定水平的体液和细胞免疫应答 ,且持续时间较长。结论 :登革病毒E基因免疫可诱发特异性免疫应答 。
Objective:To study the possibility of dengue virus E gene vaccine.Methods:The recombinant eukaryotic expression plasmid pcDNA3 E was first transfected into NIH3T3 cells by lipofectin SDS PAGE and Western blotting analyzed the expression of E gene Then the recombinant plasmid was intramuscularly injected to BALB/c mice,and the specific humoral and cellular immunity were tested Results:The recombinant plasmid DNA could induce specific immune reactions and the immune response could last a long time Conclusion:The dengue virus E gene vaccine could induce specific immune reaction,which might have provided some material and new experimential data for the further study of dengue vaccines
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2000年第12期641-643,共3页
Chinese Journal of Immunology
基金
广东省自然科学基金资助课题(5221100203017)
