摘要
目的：建立鹿衔草中没食子酸含量的测定方法。方法：采用高效液相色谱法进行测定,色谱柱：KromasilC18色谱柱（250mm×4.6mm,5μm）;流动相：甲醇-0.1%磷酸水溶液（15∶85）;检测波长：274nm;柱温：室温;流速为1.0mL.min-1;进样量：10μL。结果：没食子酸在16.28～195.36μg.mL-1的范围内与峰面积呈良好的线性关系（r=0.9995）,加样回收率为101.55%,RSD为2.50%;样品溶液在24h内稳定。结论：该测定方法简便、准确、重现性好,可用于鹿衔草药材的质量控制,为鹿衔草药材的质量评价提供参考。
Objective ： To establish a method for the determination of gallic acid in Pyrolae Herba. Methods ： HPLC was performed on Kromasil C18 column （250 mm × 4.6 mm,5 μm） at room temperature;the mobile phase consisted of carbi- nol- 0.1% phosphoric acid solution（ 15：85 ） at a flow rate of 1.0 mL min^-1, and the detection wavelength was set at 274 nm. Results： The calibration curves of gallic acid were linear in the range of 16. 28 - 195.36 μg · mL^-1 （r = 0. 9995 ）. The average recovery of quercitrin was 101.55% （ RSD = 2.50% ）, and the properties of sample solution kept steady within 24 hours. Conclusion ：This method is simple, rapid, accurate, and can be used for the quality study of the wild and cultivated Pyrolae Herba.
出处
《中华中医药学刊》
CAS
2013年第12期2794-2796,共3页
Chinese Archives of Traditional Chinese Medicine
