摘要
本研究通过识别老年男性外周血中各细胞亚群的抗原表达特点及比例,鉴别异常细胞并辅助血液系统疾病的诊断。收集88例正常老年[中位年龄82岁(70-98岁)]男性外周血,,同时标记7色荧光素抗体,采用7色流式细胞仪检测幼稚细胞、粒细胞、单核细胞、淋巴细胞、有核红细胞和浆细胞的抗原表达。按照年龄、地域和有无基础疾病分组,检验不同亚群细胞的比例是否存在差异。结果显示:老年男性外周血CD34+幼稚细胞的比例为0.017%(0.015%-0.020%),高表达HLA-DR、CD33、CD13和CD117,低表达髓系分化抗原CD15、CD11b和CD16,极少表达淋系抗原CD7、CD19和CD56;弱表达CD38,其中CD38dim+/-细胞在幼稚细胞中占(61.36±18.26)%。CD16-、CD13+CD16int+和CD16str+CD13+粒细胞在有核细胞中的中位比例分别为0.04%、0.30%和61.30%,代表了粒细胞的分化发育阶段,由不完全成熟至完全成熟。外周血中CD64+CD14-和HLA-DR+CD11b-单核细胞的比例分别为(0.00%-0.10%)和(0.07%-0.68%),为不完全成熟单核细胞。不同组中各细胞亚群比例均未见明显差别(P>0.05)。结论:进一步认识了正常老年男性外周血中CD34+幼稚细胞、不同发育阶段粒细胞和单核细胞的免疫表型特点,建立了各阶段细胞的比例范围,为鉴别异常细胞提供参考。
This study was aimed to distinguish abnormal cells and to diagnose hematologic diseases through recognizing antigen expression pattern and percentage of peripheral blood cells in normal elderly men. Antigen expression of blast cells, granulocytes, monocytes, lymphocytes, nucleated red blood cells and plasma cells was detected by seven- color flow cytometry in a total of 88 peripheral blood samples from normal elderly men, aged median 82 years old, from 70 to 98 years. Groups were divided according to age, region and underlying diseases, and the percentages of different subgroup cells were examined to confirm whether the differences were significant or not. The results showed that the median proportion of CD34＋ blast cells in peripheral blood from normal elderly men were 0. 017% （0. 015% - 0. 020% ）, with high expression of HLA-DR, CD33, CD13 and CD117, low expression of myeloid antigens, such as CD15, CDllb and CD16, while lymphoid antigens were seldom positive, including CD7, CD19 and CD56. Dim- expression of CD38 was found in peripheral blood blast cells, CD38dim＋/- cell percentage in blast cells was 61.36% ＋ 18.26%. In the differentiation and development of granulocytes, CD16-, CD13 ＋ CD16 ＋ （intermediate） and CD16 ＋ （strong） CD13 ＋ cells appeared in sequence from immature to mature granulocytes, whose median proportions in nuclear cells were 0.04%, 0. 30% and 61.30%, respectively. The percentages of immature monocytes, such as CD64 ＋ CD14- and HLA-DR＋ CD11b- cells, were from 0. 00% to 0. 10% and from 0. 07% to 0. 68%, separately. No significant differences were found between different subgroups （ P 〉 0. 05 ）. It is concluded that the immunophenotypic characteristics and referential percentages of CD34＋ blast cells, granulocytes and monocytes with different development stages in peripheral blood from normal elderly men are recognized, which can help to discriminate abnormal cells.
出处
《中国实验血液学杂志》
CAS
CSCD
北大核心
2013年第6期1585-1590,共6页
Journal of Experimental Hematology
基金
国家重大科学仪器设备开发专项(编号2011YQ03013407)
关键词
免疫表型
外周血细胞
老年男性
immunophenotype
peripheral blood cell
elderly men
