摘要
目的 探讨重组人促红细胞生成素（recombinant human erythropoietin,rh-EPO）对人乳腺癌MDA-MB-231细胞增殖的影响及其作用机制.方法 将人乳腺癌 MDA-MB-231 细胞进行培养.传至5～6代,细胞生长状态稳定后,收集人乳腺癌 MDA-MB-231细胞用于MTT实验.采用MTT法检测 5 组（阴性对照组、rh-EPO A 组、rh-EPO B组、rh-EPO C 组和rh-EPO D 组）MDA-MB-231细胞增殖的情况.用10 μmol·L-1p38MAPK抑制剂SB203580、ERK抑制剂U0126、JNK抑制剂SP600125和NF-κB 抑制剂PDTC预处理人乳腺癌 MDA-MB-231 细胞后,用MTT法检测经100、200、300和400 U·mL-1的rh-EPO（PDTC＋EPO 组、SB203580＋EPO 组、SP600125＋EPO组和U0126＋EPO组）诱导后细胞增殖的情况.结果 阴性对照组、rh-EPO A 组、rh-EPO B组、rh-EPO C 组和rh-EPO D 组 72 h PI值分别为：1.000 0±1.000 0、1.231 8±0.133 0、1.323 9±0.136 0、1.351 7±0.146 0和1.423 1±0.084 0;96 h PI值分别为：1.000 0±1.000 0、1.352 5±0.036 0、1.359 7±0.112 0、1.387 2±0.063 0和1.410 8±0.060 0.rh-EPO A 组、rh-EPO B组、rh-EPO C 组和rh-EPO D 组 72、96 h PI值与阴性对照组比较差异均有统计学意义（均P＜0.05）.PDTC＋EPO 组、SB203580＋EPO 组72、96 h PI值均较EPO组明显降低（均P＜0.05）,SP600125＋EPO组、U0126＋EPO组72、96 h PI值与EPO组比较差异均无统计学意义（均P＞0.05）.结论 rh-EPO可能是通过NF-κB、MAPK传导通路发挥效应,促进人乳腺癌MDA-MB-231细胞增殖.
Objective To investigate the effect of recombinant human erythropoietin（rh-EPO） on the proliferation of breast cancer MDA-MB-231 cells and its mechanism of action. Methods Human breast cancer MDA-MB-231 cells were cultured for 5-6 passages,and collected after steady growth was maintained. Then,cells were induced by rh-EPO at concentrations of 100,200,300 and 400 U · mL^-1 and treated with p38ERK inhibitor SB203580, ERK kinase inhibitor U0126, JNK inhibitor SP600125 and NF-κB inhibitor PDTC at concentration of 10μmol·L^-1. The proliferation of cells was detected by MTT. Results 1±. 231 8μ0. 133 0,1. 323 9±0. 136 0,i. 351 7± PI values were, respectively, 1. 000 0± 1. 0000, 0. 146 0 and 1. 423 1±0. 084 0 in negative control group, rh-EPO A group,rh-EPO B group, rh-EPO C group and rh-EPO D group at 72 hours,and 1.000 0±1.000 0,1.352 5±0.036 0,1.359 7±0. 112 0,1.387 2±0.063 0 and 1.410 8±0.060 0 at 96 hours. Pl values in negative control group were significantly different from those in rh-EPO A group,rh-EPO B group,rh-EPO C group and rh-EPO D group （P〈0.05）. Compared with EPO group,PI values significantly decreased in PDTC＋EPO group and SB203580q-EPO group at 72 and 96 hours （P%0.05）. However,PI values in EPO group were not significantly different from those in SP600125 -EPO group and U0126 ＋EPO group （P〈0.05）. Conclusion The rh-EPO can promote the proliferation of MDA-MB-231 cells through NF-κB and MAPK pathways.
出处
《南昌大学学报（医学版）》
CAS
2013年第9期1-4,25,共5页
Journal of Nanchang University：Medical Sciences
基金
福建省自然科学基金(2009J01155)
