摘要
目的:观察消癌舒(XAS)及其含药血清对Hela细胞增殖和迁移的影响,并初步探讨其作用机制。方法:采用倒置显微镜观察Hela细胞形态;四唑盐(MTT)法检测Hela细胞的增殖能力;Transwell观察Hela细胞的迁移能力;酶联免疫吸附实验(ELISA)检测Hela细胞糖类抗原125(CA125)的水平;real time RT-PCR法检测Hela细胞血管内皮生长因子(VEGF)mRNA的表达。结果:经消癌舒处理后Hela细胞形态发生了改变,表现为细胞折光度下降,体积明显缩小,形状变圆,消癌舒及其含药血清均明显抑制Hela细胞的增殖,消癌舒减少Hela细胞穿过滤膜的个数,降低Hela细胞CA125水平及VEGF mRNA的表达,且与顺铂联用效果更佳。结论:消癌舒及其含药血清明显抑制Hela细胞的增殖及迁移能力,与顺铂联用作用更明显,其机制可能与降低CA125水平和VEGF mRNA的表达有关。
Objective： To observe the effect of Xiaoaishu（XAS） and serum containing XAS on proliferation and migration of human cervical carcinoma Hela cell in vitro, and investigate the mechanism. Method： The morphology of Hela cell was observed by inverted microscope. Thiazolyl blue （MTT） assay was used to detect Hela cell proliferation. Migration ability of Hela cells was measured through transwell assay. The carbohydrate antigen 125 （CA125） level of Hela cell was measured by enzyme linked immunosorbent assay （ELISA）. Real time RT-PCR was used to detect the expression of vascular endothelial growth factor （VEGF） mRNA. Result： The morphology of Hela cell was changed after XAS treatment, the main expressions of Hela cell consisted in decreased cell diopter, turned round and small. The proliferation and migration of Hela cells was significantly inhibited by XAS and serum containing XAS. The level of CA125 and the expression of VEGF mRNA were decreased after XAS treatment, and XAS combined with Cisplatin had a better effect. Conclusion： XAS and serum containing XAS had obvious effect against the proliferation and migration of Hela cell, which showed much higher superiority to the single medication group combined with cisplatin, and it＇s mechanism may be related with the reduction of CA125 level and VEGF mRNA expression.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第24期250-254,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
贵州省科技厅中药现代化专项(黔科合中药字20115008号)
贵州省教育厅自然科学类科研项目(黔教科2011056号)
贵州省中医药管理局项目(D274,QZYY2010-59)
贵州省科技厅自然科学基金(黔科合J字20092147号)
