摘要
为了研究沙门氏菌多重耐药性与Ⅰ型整合子的流行情况,本研究从山东省鸡场分离沙门氏菌,根据Kauffmann-White方法测定其血清型,采用微量稀释法测定了沙门氏菌对19种常用抗菌药物的MIC值,并且采用PCR技术测定了Ⅰ型整合子及其携带的耐药基因盒。结果显示:本次分离得到311株沙门氏菌,包括印地安纳沙门氏菌(133株)和肠炎沙门氏菌(178株)2种血清型;药物敏感性试验表明:分离菌株对磺胺嘧啶、磺胺甲恶唑、萘啶酸、氨苄西林、四环素、多西环素和甲氧苄啶普遍耐药,菌株多重耐药率为91.0%(283/311),99%的分离株对阿米卡星、多黏菌素敏感;PCR测定Ⅰ型整合子结果:Ⅰ型整合子的检出率为65.0%(202/311),其中多重耐药表型菌株Ⅰ型整合子阳性率为92.6%,202株整合子阳性菌株中有6株的携带基因盒dfr17-aadA5。上述结果表明,Ⅰ型整合子普遍存在于沙门氏菌中,并且与沙门氏菌的多重耐药性密切相关。
In order to study the multiple drug resistance of Salmonella and integron pop, this article from the Shandong Province farm for isolation of Salmonella species, according to the Kauffmann-White method for the determination of serum type, by microdilution method for the determination of the Salmonella 19 antimicrobial drugs against MIC, and using PCR technique for the determination of the class I integrons and carrying resistance gene cassette. The results showed, the isolated from 311 strains of Salmonella, including Indiana Salmonella （133 lines） and Salmonella Enteritidis （178 lines） in 2 serum type. Drug sensitivity test showed： isolated bacterial strains to sulfamethoxazole, sulfadiazine, nalidixic acid, ampicillin, tetracycline, doxycycline and trimethoprim resistance strains generally, multiple drug resistance rate was 91% （283/311）, 99% of the isolates to Amikacin, polymyxin sensitive. PCR determination of integron results： class I integron detection rate was 65% （202/311）, in which multiple phenotypic resistance strain type I integron positive rate was 92.6%, 202 isolates integrons positive strains in 6 strains carrying dfrl7-aadA5 gene cassette. The results showed that, class I integron exists generally in Salmonella and related to multiple drug resistance.
出处
《中国农学通报》
CSCD
2013年第32期39-43,共5页
Chinese Agricultural Science Bulletin
基金
国家自然科学基金"动物源沙门氏菌耐药基因岛变异及传播机制"(31201949)
北京农学院科研质量提高经费资助"沙门氏菌多重耐药基因传播机制"(GJB2012003)
关键词
沙门氏菌
多重耐药性
整合子
基因盒
Salmonella
multidrug resistance
class I integron
gene cassette
