摘要
[目的]探讨失血性休克复苏后早期胃内灌注ω-3多不饱和脂肪酸(ω-3PUFAs)对大鼠肠黏膜屏障功能的影响。[方法]选取健康成年SD大鼠72只,按照胃内灌注状况,随机分为ω-3PUFAs组、牛奶组、不干预组。乌拉坦腹腔麻醉后,制作休克大鼠模型,休克30 min后复苏,ω-3PUFAs组和牛奶组在复苏结束、复苏2h分别进行胃内灌注。各组分别在休克30min、复苏2h和4h处死1/3大鼠,取肝脏组织进行细菌培养,取一段空肠制成病理切片。[结果]肝脏组织细菌培养显示,ω-3PUFAs组、牛奶组复苏2h、4h菌落数较休克30min少,同时限点也较不干预组少(P<0.05);肠道病理切片显示,复苏2h、4hω-3PUFAs组较牛奶组和不干预组轻,不干预组损伤最重。[结论]失血性休克救护过程中,早期胃内灌注ω-3PUFAs更有利于肠道屏障功能的恢复、减少肠内细菌移位。
Abstract Objective：To probe into influence of early intragastric perfusion of ω- 3 PUFAs in hemorrhagic shock rats after resuscitation on intestinal barrier function of hemorrhagic shock in rats. Methods： Sprague - Dawley rats were randomly divided into 3 group in accordance with the intragastric perfusion：ω- 3PUFAs group.milk group and the control group. After in- traperitoneal urethane anesthesia, shock rats models were produced, resus- citation was carried out for them after shock for 30 min. The models in o^- 3PUFAs group, milk group received intragastrical infusion respectively at the end of resuscitation and at two hours after resuscitation. Each 1/3 of rats in each group were killed at 30 minutes after shock and at 2 and 4 hours after resuscitation, separately. The liver tissue was taken for bacterial culture and a segment of jejunal was taken to make pathological section. Results：Liver tissue cultures showed that,the number of colonies in ω - 3 PUFAs group and milk group at 2 and 4 hours after resuscitation was less than that at 30 min after shock, and was less than that in non -intervention group at the same time （P〈0.05） ;intestinal biopsy showed that the dam- age of ω - 3 PUFAs group was lighter than that of milk group and non - in- tervention group at 2 and 4 hours after resuscitation, the damage was the most serious in non - intervention group. Conclusion：During ambulance of hemorrhagic shock,early intragastric infusion of ω- 3PUFAs is more con- ducive to the recovery of intestinal barrier function and reducing intestinal bacterial translocation.
出处
《护理研究（上旬版）》
2013年第12期3865-3867,共3页
Chinese Nursing Researsh
基金
2012年国家级大学生创新创业计划项目
编号:201290035033
关键词
大鼠
失血性休克
Ω-3多不饱和脂肪酸
胃内灌注
肠黏膜屏障
细菌移位
rats
hemorrhagic shock
ω- 3 polyunsaturated fatty acids
in-tragastric infusion
intestinal mucosal barrier~ bacterial translocation
