摘要
目的探讨汉族人群血清载脂蛋白A5(APOA5)水平及其-1131T/C位点基因多态性与早发冠心病及经皮冠状动脉介入治疗(PCI)术后支架内再狭窄的关系。方法选取2008年9月—2011年8月在长沙市中心医院住院的早发冠心病患者170例,根据冠状动脉病变程度分为主要血管病变<75%组40例(行药物保守治疗),主要血管病变≥75%组50例(行PCI术),急性冠脉综合征(ACS)组80例(行PCI术);另选取同期在长沙市中心医院保健门诊体检正常者158例作为对照组。采用全自动生化分析仪测定所有受检者血清总胆固醇(TC)、三酰甘油(TG)、空腹血糖(FPG)、低密度脂蛋白(LDL)、高密度脂蛋白(HDL)水平,酶联免疫吸附试验(ELASA)测定血清APOA5水平,聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)法测定APOA5-1131T/C基因型。所有行PCI术患者1年后复查冠状动脉造影(CAG),根据复查结果分为支架内再狭窄组和支架内无狭窄组,同时再次检测其血清学指标及APOA5-1131T/C基因多态性。结果对照组、主要血管病变<75%组、主要血管病变≥75%组、ACS组血清TC、LDL、FPG水平比较,差异均无统计学意义(P>0.05);血清TG、HDL、APOA5水平比较,差异均有统计学意义(P<0.05)。主要血管病变<75%组、主要血管病变≥75%组、ACS组血清TG水平高于对照组,而血清HDL、APOA5水平低于对照组(P<0.05)。各组APOA5-1131T/C基因型及等位基因频率比较,差异均有统计学意义(P<0.05)。所有行PCI术患者复查CAG发现支架内再狭窄30例,支架内无狭窄100例,两组血清TC、TG、LDL、HDL、FPG、APOA5水平比较,差异均无统计学意义(P>0.05),APOA5-1131T/C基因型及等位基因频率比较,差异均有统计学意义(P<0.05)。结论血清APOA5水平及APOA5-1131 C等位基因与早发冠心病及PCI术后支架内再狭窄有关,而APOA5-1131 C等位基因可能为预测早发冠心病及PCI术后支架内再狭窄的危险因素。
Objective To investigate the relationship of apolipoprotein A5 （APOA5） and its - 1131T/C locus gene polymorphism to early - onset coronary heart disease （CHD） and restnosis after percutaneous coronary intervention （PCI） in Han population. Methods A total of 170 early - onset CHD patients hospitalized in this hospital from September 2008 to Au- gust 2011 were divided, according to degrees of coronary artery lesions, into groups A （artery lesion 〈 75%, given conservative therapy, n=40） , B （artery lesions〉75% , given PCI, n =50） , C （with acute coronary syndrome, given PCI, n =80） , and 158 healthy controls having physical examinations were enrolled as control group. Automatic biochemical analyzer were used to determine the levels of serum total cholesterol （TC） , triglyceride （TG） , fasting plasma glucose （FPG）, low density lipo- protein （LDL）, high density lipoprotein （HDL）; Enzyme- linked immunosorbent assay （ELASA） to detect serum APOA5 level; Polymerase chain reaction- restriction fragment length polymorphism （PCR- RFLP） to determine APOA5 -1131T/C genotype. All patients having had PCI were rechecked by coronary angiogrphy （CAG） to check the in - stent restenoses after 1 year. Serum indicators and APOA5 - 1131T/C gene polymorphism were detected. Results There was no significant difference in TC, LDL, FPG levels in groups control, A, B, C （P 〉 O. 05）, but there was in TG, HDL, APOA5 levels （P 〈 0. 05）. The TG level was higher, levels of HDL, APOA5 lower in groups A, B, C than those in control group （P 〈0. 05）. In all PCI patients rechecked by CAG, 30 had in -stent restenoses, 100 had no. There was no significant difference in levels of TC, TG, LDL, HDL, FPG, APOA5 between 2 groups （P 〉0.05）, but there was in APOA5 - 1131C genotype and allele frequency （P 〈 0.05 ）. Conclusion APOA5 level and APOA5 - 1131 C alleles are related to early - onset CHD and post - PCI in - stent rest- enoses. APOA5 - 1131C alleles may be a risk fa
出处
《中国全科医学》
CAS
CSCD
北大核心
2013年第32期3821-3824,共4页
Chinese General Practice
关键词
载脂蛋白A类
多态性
单核苷酸
冠心病
血管成形术
经腔
经皮冠状动脉
冠状动脉再狭窄
Apolipoproteins A
Polymorphism, single nucleotide
Coronary disease
Angioplasty, transluminal, percutaneous coronary
Coronary restenosis
