摘要
目的:建立蒙药土茯苓七味汤散中栀子苷的含量测定方法。方法:采用高效液相色谱法(HPLC),应用Diamonsil C18(250mm×4.6mm,5μm);流动相为甲醇-0.1%磷酸溶液(25:75);流速为1.0mL/min;检测波长为238nm;柱温为25℃。结果:栀子苷在0.1075-0.6450μg范围内线性关系良好(r=0.9999),栀子苷的平均回收率为97.30%、RSD为1.40%(n=6)。结论:含量测定方法简便,精密度高,分离度好,可用于蒙药土茯苓七味汤散中栀子苷的含量测定。
Objective： To establish the content determination methods of geniposide in Mongolian medicine Poria Seven Tastes Soup Powder. Methods： The geniposide content was determined by high performance liquid chromatography （HPLC） with Diamonsil C18 （250mm×4.6mm, 5μm） chromatographic column at 25℃. The mobile phase was methanol and 0.1% phosphoric acid （25 ： 75） with flow rate of 1.0 mL/min. The detection wavelength was 238 nm. Results： The linear relationship for geniposide was good in the range of 0.1075-0.6450 lag （r=-0.9999）. The average recover was 97.30% and RSD was 1.40% （n=6）. Conclusion： The content determination is ease, with high accuracy and good resolution, which can be applied to the content determination of geniposide in Mongolian medicine Poria seven tastes soup powder.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2013年第12期3666-3668,共3页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
内蒙古民族大学蒙药研发工程实验室创新能力建设项目(No.2009FGW010)~~
