摘要
目的 观察复方调视膏皮肤用药的安全性,为防治青少年近视眼临床安全用药提供科学实验依据.方法 实验1,采用家兔进行皮肤急性毒性试验,分为完整皮肤组和破损皮肤组,同时各组分高、低剂量组和赋形剂对照组（共5组）,分别于给药24 h后,去除残留的药物,去除药物后1h、24 h、48 h、72 h至第7天,每天观察上述各组是否引起家兔皮肤急性毒性反应.实验2,采用同体家兔左右侧自身对比进行皮肤刺激性试验,分完整皮肤及破损皮肤组,同时各组分高、中、低剂量组和同体对侧相同剂量赋形剂对照6组,观察各组多次给药后所产生的局部刺激反应,并在连续给药7天后,取给药皮肤组织进行病理组织学检查.实验3,采用豚鼠进行皮肤过敏性试验,分为赋形剂组、调视膏组、及阳性对照3组,分别于第6h、7d、14d进行右侧去毛皮肤致敏试验;于第28天,进行左侧去毛皮肤激发试验.观察各组是否引起豚鼠皮肤或全身过敏反应.结果 实验1,受试各组均未引起皮肤急性毒性反应;实验2,家兔皮肤刺激性试验结果,完整皮肤组肉眼观察无红斑、水肿等现象,破损皮肤组在1～5d内均出现红斑、水肿,分别与相应时间赋形剂对照组比较,差异均无统计学意义,P均＞0.05.家兔破损皮肤组在1～5 d内出现的红斑、水肿现象是由于皮肤损伤引起的炎症反应所致,非用药所致,病理组织学检查均未见异常;实验3,皮肤过敏试验赋形剂和调视膏组未引起豚鼠皮肤过敏反应和全身过敏反应.结论 复方调视膏动物试验,未引起皮肤急性毒反应和皮肤刺激性以及皮肤过敏反应.
Objective To observe the safety of the compound Tiaoshi-plaster＇s use to skin,providing the scientific foundation for its use to the treatment for the younth＇s short sight.Methods Experiment 1,acute toxicity test for skin of rabbits：rabbits were randomly divided into two groups：a integrated skin group and a damaged skin group,in addition,every group further divide into high and low dosage group and vehicle control group,totally 5 groups.All above groups received the plaster or vehicle for 24 hours,then take away the drug,and after lh,24 h,48 h,72 h since the 7th day,observe each group＇s reaction separately everyday,to see whether there is a acute toxicity reaction or not.Experiment 2,self control group,use onerabbit＇s left and right sides＇ skin to observe stimulus toxicity via skin：divide the rabbits into a integrated skin group and a damaged skin group,in addition,every group further divide into high,low dosage group and vehicle control group,totally 6 groups.Observe the reaction after multiple doses,after 7 days＇ successive doses,take the drug-used tissues to do pathological examination.Experiment 3,cavy＇s skin irritation test：cavies were divide into vehicle group,plaster group,positive group,totally 3 groups,at the 6h,7th day,14th day,take off right side＇s skin of every group to do the provocative test respectively.On the 28th day,take off the left side＇s skin to do the provocative test.To see if the skin or the whole body is allergic to the plaster.Results In experiment 1,toxic response could not be found in any group; In experiment 2,rabbits in the integrated skin group didn＇t show redness or edema,rabbits in the damaged skin group all had redness and edema,but the differences were not statistically significant,compared with the corresponding time of vehicle group （P〉0.05）.Such skin damage was not caused by plaster usage but inflammatory reaction of skin damage.In experiment 3,the vehicle group and the study group didn＇t show any abnormal anaphylactic reaction.Conclusion T
出处
《国际中医中药杂志》
2013年第12期1086-1089,共4页
International Journal of Traditional Chinese Medicine
基金
2011年度内蒙古治自区卫生厅科研课题：复方调视膏防治青少年近视眼皮肤用药安全性试验研究
关键词
复方调视膏
青少年近视眼
皮肤急性毒性试验
皮肤刺激性试验
皮肤过敏性试验
Compound Tiaoshi-plaster
Younth＇s short sight
Acute toxicity test for skin
Provocative test for skin
Anaphylactic test for skin
