摘要
目的探讨风湿清方对胶原诱导的关节炎(collagen-induced arthritis,CIA)大鼠骨保护作用机制。方法试验分为空白组、模型组、西药组(MTX,1 mg/kg)和中药组(风湿清方,24 g生药/kg),除空白组外,其余各组大鼠CIA造模,西药组与中药组免疫后第14天开始给药,给药直至第56天。观察各组大鼠足趾体积(每周二、周五测量),检测干预3、6周时血清IL-17、RANKL、MIP-1α的表达水平及骨密度、核素骨显像,观察病理切片,评价清热活血方药对CIA大鼠骨破坏的干预情况。结果造模各组第10天起,双足体积开始增加,21天左右达到高峰,30天左右明显下降,其中两个给药组比模型组肿胀程度轻。与模型组同期比较,西药组干预3周后IL-17、RANKL降低(P<0.01,P<0.05),中药组干预3周后IL-17降低(P<0.05);两个给药组干预6周后RANKL均降低(P<0.01,P<0.05)。与模型组及西药组同期比较,中药组干预6周后整体BMD及踝关节BMD均升高(P<0.01,P<0.05).,中药组干预3周踝ROI/下颌骨升高、足趾ROI/下颌骨升高(P<0.05)。病理切片发现模型组关节腔隙明显增宽,滑膜组织增生严重,骨组织破坏,与模型组比较,两个给药组改善显著,软骨结构轮廓完整。结论风湿清方可以抑制骨密度下降,防止骨破坏,其机制可能通过骨免疫Th/RANKL系统,下调IL-17、RANKL、MIP-1α的表达水平,抑制破骨细胞的成熟和分化,促进成骨细胞活跃,从而抑制骨破坏。
Objective To explore the protective mechanism of Fengshiqing Recipe （FR） against bone destruction in collagen-induced arthritis （CIA） rats. Methods Rats were divided into four groups in the experiment,i.e., the blank control group,the model group,the MTX group（MTX, 1 mg/1 000 g）,and the FR group （24 g crude FR/kg）. The CIA model was prepared except the blank control group. Medication was started in the MTX group and the FR group from the 14th day after modeling to the 56th day. The toe volume was measured on every Tuesday and Friday. Expression levels of serum IL-17, RANKL, MIP-1α were detected after 3-and 6-week intervention. The bone scintigraphy with nuclide （SPECT）, bone mineral density （BMD）, and the pathological section were observed to assess the intervention of drugs of heat clearing blood activating actions in the bone destruction of CIA rats. Results From the 10th day of modeling, the volume of both toes started to swell and reached the peak at about 21 days. It was obviously shrunk at about 30 days. Of them, the swelling degree was milder in the MTX group and the FR group than in the model group. Compared with the model group at the same phase, the levels of IL-17 and RANKL decreased in the MTX group after 3 weeks of intervention （P 〈0.01, P 〈0.05）. The IL-17 level decreased in the FR group after three weeks of intervention （P 〈0.05）. The RANKL level decreased in the MTX group and the FR group after 6 weeks of intervention （P 〈0.01, P 〈0.05）. Compared with the model group and the MTX group, the overall BMD and ankle BMD increased in the FR group after 6 weeks of intervention （P 〈0.01, P 〈0.05）. The ankle ROI/mandible and the toe ROI/mandible were elevated in the FR group after 3 weeks of intervention （P 〈0.05）. Pathological results suggested that the joint lacune was significantly widened, the hyperplasia of the synovial tissue was so severe, and the bone tissue was de- stroyed in the model group. Compared with the model group, the aforesaid
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2013年第12期1648-1652,共5页
Chinese Journal of Integrated Traditional and Western Medicine
基金
国家自然科学基金资助项目(No.30873250)
关键词
类风湿关节炎
风湿清方
骨免疫学
核素骨显像
骨密度
rheumatoid arthritis
Fengshiqing Recipe
osteoimmunology
bone scintigraphy with nuclide （SPECT）
bone density
