摘要
目的研究不同分离来源阿萨希毛孢子菌（Trichosporon asahii，T．asahii）的体外药敏及致病性差异，为临床资料提供参考依据。方法用E—test法测定6种常用抗真菌药物对12株不同来源T．asahii的MIC值，评估它们的体外药物敏感性差异；通过动物实验计算7株不同来源T．asahii的半数致死量，评估其致病性差异。结果12株T．asahii对氟胞嘧啶、阿尼芬净均先天耐药，对两性霉素B具有不稳定性，对唑类药物较敏感，其中对伏立康唑最敏感；环境株较临床株对唑类药物更敏感；致病性结果：临床株的致病性显著高于环境株，它们的半数致死量较环境株高1～3个数量级，其中BZP07005的半数致死量最高，CBS7]37的半数致死量最低；环境分株和临床分离株自身之间的半数致死量也有差异。结论T．asahii临床株与环境株的体外药物敏感性和致病性差异均有统计学意义，环境株的药敏性更高，致病性更低。
OBJECTIVE To observe the in vitro drug susceptibility and pathogenicity of the Trichosporon asahii （T. asahii） strains isolated from different specimen sources so as to provide evidence for the clinical data. METHODS The E-test was performed to determine the MIC value of 12 isolates of T. asahii to 6 commonly used antibiotics, then the in vitro drug susceptibilities were assessed, the median lethal dose of 7 strains of T. asahii was calculated through the animal experiment, and the pathogenicity was evaluated. RESULTS The 12 isolates of T. asahii were naturally resistant to flucytosine and anidulafungin, with instable susceptibility to amphotericin B, yet the strains were susceptible to azoles, voriconazole in particularly; the environmental strains were more sus- ceptible to the azoles than the clinical strains. As for the pathogenicity, the pathogenicity of the clinical strains was significantly higher than that of the environmental strains, the clinical strains had 1 to 3 magnitude orders of medi- an lethal dose greater than the environmental strains, the median lethal dose of BZP 07005 was the highest, CBS 7137 the lowest. There was also difference in the median lethal dose among the environmental strains or the clini- cal strains themselves. CONCLUSION The differences in both the in vitro drug susceptibility and the pathogenicity between the clinical T. asahii strains and the environmental strains are statistically significant, and the environmental strains have higher drug susceptibility and lower pathogenicity.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2013年第24期5915-5917,共3页
Chinese Journal of Nosocomiology
基金
军队"十二五"重点基金资助项目(BWS11J059)
关键词
T
asahii
不同来源
体外药敏性
致病性
T. asahii
Different sources
In vitro drug susceptibility
Pathogenicity
