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异黏蛋白在乳腺癌细胞中的表达 被引量:3

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摘要 异黏蛋白(MTDH)作为一个癌基因,可在全身多种肿瘤中过表达[1].MTDH的过表达可能与乳腺癌的耐药和转移有关.本研究旨在测定4种乳腺癌细胞中MTDH的表达水平,探讨MTDH的表达与乳腺癌细胞生物学特性之间的关系.一、材料与方法1.材料:乳腺癌细胞:MDA-MB-231、MCF-7、 MDA-MB-435S、 MCFG/ADR.TransStart Top Green qPCR Supermix,逆转录试剂盒,MTDH鼠抗人单克隆抗体.2.实时定量聚合酶链反应(Realtime PCR):抽提细胞RNA合成cDNA,进行聚合酶链反应.内参基因为甘油醛-3-磷酸脱氢酶(GAPDH),内参引物和MTDH引物参照文献[2].ABI7500仪器设置为SYBR Green法,计算2-△△Ct,进行统计分析.
出处 《中华实验外科杂志》 CAS CSCD 北大核心 2013年第12期2690-2690,共1页 Chinese Journal of Experimental Surgery
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同被引文献25

  • 1陈曦,孙海斌.组织蛋白酶D、Tmem 16a和乳腺肿瘤激酶在结肠癌中的表达及意义[J].中国老年学杂志,2014,34(7):1984-1985. 被引量:4
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