摘要
为了研究苜蓿总黄酮对动物妊娠期间繁殖性能、繁殖器官、激素水平及下丘脑-垂体-卵巢性腺轴相关基因表达量的影响,本实验以雌性大鼠为模式动物,分为5个试验组:3个苜蓿总黄酮剂量组(120,400,1200mg/kg)和2个对照组(己烯雌酚0.5mg/kg、空白对照组),结果显示,苜蓿总黄酮中、高剂量组雌二醇(E2)显著升高(P<0.05),低剂量组E2极显著升高(P<0.01)。各试验组的血清中孕酮(P)含量不存在显著性差异(P>0.05)。苜蓿总黄酮3个剂量组的活胎数均显著提高(P<0.05),高剂量组的胎仔重极显著增重(P<0.01),而苜蓿总黄酮各剂量组的黄体数差异不显著,但存在随着剂量的增加而降低的趋势,己烯雌酚的黄体数与空白对照组相比显著减少(P<0.05)。苜蓿总黄酮3个剂量组的产仔数均显著高于空白对照组(P<0.05),而初生窝重则极显著高于对照组(P<0.01)。苜蓿总黄酮和己烯雌酚均显著降低下丘脑中GnRH的表达量(P<0.01),同时垂体的FSH表达量显著降低(P<0.01),卵巢中苜蓿总黄酮高剂量组的FSHR表达量显著降低(P<0.05),LHR表达量显著升高(P<0.05),其他基因表达量与空白对照相比差异不显著(P>0.05)。从以上可以看出,苜蓿总黄酮通过影响下丘脑-垂体-卵巢性腺轴,改善了妊娠期间雌鼠的繁殖性能,提高了产仔数和初生窝重。
This study aimed to elucidate the effects of alfalfa flavonoids on reproductive performance, reproduc tive organs, hormone level, and gene expression in the hypothalamus-pituitary-ovarian gonadal axis in pregnant animals. Three doses of alfalfa flavonoids （120, 400 and 1200 mg/kg） and two control groups （diethylstilbes trol 0.5 mg/kg, blank control （P〈0.05） increased the level g of roup） were used. The middle and high doses of alfalfa flavonoids significantly estradiol （E2） （P〈0.05）, while the low dose of alfalfa flavonoids highly sig nificantly （P〈0.01） increased the level of E2. There was no evident difference of progesterone level between the groups. The number of live fetus significantly （P〈0.05） increased in three doses of alfalfa flavonoids, the weight of live fetus was highly significantly （P〈0.01） increased with the high dose of alfalfa flavonoids. There was no evident difference in the number of luteum although there was a declining trend. Moreover, compared with control group, diethylstilbestrol, reduced the number of luteum. Compared with the control group, the three doses of alfalfa flavonoids significantly （P〈0.05） increased the litter size, and the newborn litter weight increase was highly significant （P〈0.01）. Expressions of GnRH in the hypothalamus and FSH in the pituitary were highly significantly （P〈0.01） reduced by alfalfa flavonoids and diethylstilbestrol. The expression of FSHR in the ovaries was reduced by the high dose of alfalfa flavonoids, but the expression of LHR was increased. The expressions of other genes showed no significant difference compared with the control group. In conclusion, the reproductive performance of rats was improved, because the alfalfa flavonoids influenced the hypothalamus-pituitary-ovarian gonadal axis.
出处
《草业学报》
CSCD
北大核心
2013年第6期249-256,共8页
Acta Prataculturae Sinica
基金
国家"十二五"科技支撑计划(2011BAD17B04)
河南省杰出青年基金(114100510022)
国家牧草产业技术体系(nycytx-35)资助
关键词
苜蓿总黄酮
下丘脑-垂体-卵巢性腺轴
初生窝重
产仔数
大鼠
荧光定量
alfalfa flavonoids
hypothalamus-pituitary-ovarian gonadal axis
newborn litter weight
litter size
female rat
qPCR
