摘要
目的 建立一种红芪多糖含量测定的方法.方法 采用80%乙醇回流除去蛋白、氨基酸、单糖、双糖、低聚糖等杂质,并用热水分离提取红芪多糖,以葡萄糖作为换算因子,绘制标准曲线,用蒽酮-硫酸分光光度法对红芪多糖含量进行测定.结果 检测波长为625nm,得到葡萄糖标准曲线回归方程为y=6.3263＊c＋0.1271,R2=0.9917,线性范围为0～0.2mg/ml.该测定方法供试液在2h内显色稳定,精密度、稳定性和重复性良好,平均回收率100.53%,RSD〈3.0%,测得红芪多糖的含量为6.62%.结论 蒽酮-硫酸法操作简便,重现性好,准确,可靠,可作为红芪多糖的测定.
Abstract：Objective To establish a radix hedysari Polysaccharide determination method. Methods Radix hedysari polysaccharide was extracted by 80% ethanol to remove out protein, amino acids, monosaccharides, disaccharides, oligosaccharides and other impurities, and the polysaccharide used glucose as a conversion factor, then obtained the standard curve. The polysaccharide was determined with anthrone-sulfuric acid spectrophotometry. Results The detection wavelength was 625nm, and the glucose standard curve regression equation was y = 6. 3263＋O. 1271 ,R2 = 0. 9917, and linear range was 0-0.2rng/ml. The method was simple and feasible for the color stability of test solution within 2h. The precision and stability was good. The average recovery was 100. 53 %, and all RSD〈3.0 %. Thc polysaccharidc content in radix hcdysari is 6. 62 %. Conclu sion The anthrone-sulfuric acid method is simple, reproducible, accurate and reliable, which can be preferably used as the method of determi-nation of radix hedysari polysaccharide.
出处
《中国中医药现代远程教育》
2013年第20期149-151,共3页
Chinese Medicine Modern Distance Education of China
关键词
红芪
多糖
含量
测定
Radix hedysari, Polysaccharides
Content Determination
