摘要
目的探讨基质金属蛋白酶1、3（MMP．1、MMP．3）及其组织抑制因子1、3（TIMP-1、TIMP．3）在结膜松弛症（CCh）成纤维细胞培养上清液中的表达，揭示四者表达水平变化与CCh的内在联系。方法体外原代培养CCh成纤维细胞，取第3—6代细胞，以2．5×10^5个／mL浓度接种于24孔板中培养，24h后提取细胞上清液，采用ELISA检测MMP-1、MMP-3、TIMP-1、TIMP-3的表达水平。不同组间比较采用单因素方差分析。结果MMP-1的表达：CCh组成纤维细胞的蛋白浓度明显高于正常对照组，差异有统计学意义（P〈0．05）。MMP-3的表达：CCh组成纤维细胞的蛋白浓度明显高于正常对照组，差异有统计学意义（P〈0．01）。TIMP．1的表达：CCh组成纤维细胞的蛋白浓度与正常对照组比较，差异无统计学意义（P〉0．05）。TIMP-3的表达：CCh组成纤维细胞的蛋白浓度明显低于正常对照组，差异有统计学意义（P〈0．05）。结论成纤维细胞MMP．1／TIMP-1及MMP-3／TIMP-3的表达失衡可能参与了CCh的发生、发展。
Objective To investigate the expression of MMP-1, MMP-3, TIMP-1 and TIMP-3 in the cell culture supernatant of fibroblasts of Conjunctivochalasis. To reveal the internal link between the expression level changes of four indicators with CCh . Methods Three to six passage cells were taken from primitive culture CCh fibroblasts in vitro. They were cultured with concentration of 2. 5 ×10^5/mL in 24 hole culture plate cultivation. Cell supernatant was extracted after 24 h. ELISA method was used to detect the expression of MMP-1, MMP-3, TIMP-1 and TIMP-3. Single factor analysis of variance was used to compare the difference among groups. Results The expression of MMP-1 ： the protein concentrations of CCh fibroblasts were significantly higher than that of normal control group （ P 〈 0. 05 ）. The expression of MMP-3 ： the protein concentrations of CCh fibroblasts were significantly higher than the normal control group （P 〈 0.01 ）. The expression of TIMP-1 ： there was no statistically significant difference （ P 〉 0.05 ） of the protein concentration of between CCh fibroblasts with the control group. The expression of TIMP-3 ： the protein concentration of CCh fibroblasts is significantly lower than that of normal control group （P 〈 0.05 ）. Conclusions The expression of MMP-1/TIMP-land MMP-3/TIMP-3 imbalances may participate in the development of the CCh.
出处
《中国眼耳鼻喉科杂志》
2013年第6期365-367,共3页
Chinese Journal of Ophthalmology and Otorhinolaryngology
基金
上海市卫生局科研基金资助项目(20124415)
