摘要
目的建立高效液相色谱法测定维生素B1片中维生素B1的含量。方法采用色谱柱：Diamonsil C18（150mm×4.6mm,5μm）,以碳十八烷基键合硅胶为填充剂。流动相：甲醇-乙腈-0.02mol/L庚烷磺酸钠溶液（含1%三乙胺,用磷酸调节pH值至5.5,9∶9∶82）;检测波长为238nm;流速为1.0ml/min;进样量：10μl。结果维生素B1质量浓度在50.5~505μg/ml范围内与峰面积呈良好的线性关系（r=0.99999,n=5）,平均回收率为100.4%,RSD=0.13%。结论本方法简单、精确、可靠,可作为维生素B1片的质量控制方法。
Objective To establish HPLC method for the determination of Vitamin B1Tablets.Methods Using Column：Diamonsil C18（15 0 m m×4. 6 m m, 5μm）, Mobi le phase：methanol-acetonitrile-0.02mol/L sodium heptane solution（containing 1%triethylamine,pH value adjusted to 5.5 with phosphoric acid,9∶9∶82）.The flow rate was1.0ml/min.The detection was carried out with an ultraviolet detector at 238nm.Thiamine hydrochloride was measured with the external standard method.Results The calibration curve was linear in the range of 50.5-505μg/ml.（r=0.99999,n=5）,the average recovery rate was 100.4%,RSD=0.13%.Conclusion This method is simple,convenient,accurate and reliable.It can be used to control the quality of Vitamin B1 Tablets.
出处
《首都医药》
2013年第22期71-72,共2页
Capital Medicine
关键词
高效液相色谱法
维生素B1
含量测定
High Performance Liquid Chromatography
Vitamin B1
Content Determination
