摘要
利用已知的芜菁HY5编码基因序列为探针,采用电子克隆的方法获得了油菜HY5基因cDNA的序列。采用生物信息学工具对其编码蛋白序列进行了研究,并将其编码蛋白同芜菁和拟南芥的HY5蛋白进行了同源性比较,构建了其系统发育树。结果表明:油菜HY5基因编码164个氨基酸,其分子量为18 037.8Da,是亲水蛋白,同芜菁HY5具有高度的同源性,高于和拟南芥的同源性;该试验初步获得了油菜HY5基因的编码序列,为进一步研究其功能打下基础。
With HY5 gene in Brassica rapa as probe, the cDNA of HY5 gene of Brassica napus was in silico cloned. Using bioinformaticstool,amino acid sequence coded by HY5 of Brassica napus was predicted and homology compared with Arabidopsis thaliana and Brassica rapa. Its phylogenetie tree was constructed. The results showed that HY5 of Brassica napus coded a protein of 164 amino acids. The calculated molecular weight of it was 18 037. 8 Da. It was a hydrophilic protein. The homology of HY5 protein between Brassica napus and Brassica rapa was higher than that between Brassica napus and Arabidopsis thaliana. The HY5 gene coding sequence of Brassica napus was cloned preliminary,and laid the foundation for further study its function.
出处
《北方园艺》
CAS
北大核心
2013年第23期116-118,共3页
Northern Horticulture
基金
河北省高等学校自然科学研究计划资助项目(Z2012045)
河北省科学技术研究与发展计划资助项目(12212702)
关键词
HY5
油菜
光受体
电子克隆
HY5
Brassica na pus
photoreceptor
in sil ico cloning
