摘要
以山蜡梅、"金蓓"素心蜡梅及"扬州黄"素心蜡梅为试材,使用扫描电子显微镜观察,研究了氯仿加热不同时间处理对蜡梅盛开期花被片蜡质提取效果的影响。结果表明:山蜡梅花被片蜡质提取最适条件为氯仿60℃处理30s,表层蜡质含量约为1 034.33μg/cm2;素心蜡梅"金蓓"和"扬州黄"花被片蜡质提取最适条件均为氯仿60℃处理20s,"金蓓"素心蜡梅花被片表层蜡质含量约为651.33μg/cm2,"扬州黄"素心蜡梅花被片表层蜡质含量约为1 057.33μg/cm2。
Taking Chirnonanthus nitens Oliv, Chimonanthus praecox ＇ Jinbei ＇ and Chirnonanthus praecox ＇ Yangzhouhuang ＇ as meterials, the treated samples were observed by scanning electron microscope （SEM）for establishing the method of the petal surface wax extraction during their full bloom period by using hot chloroform treatments. The results showed that the treatment with chloroform of 60℃ for 30 s was optimal for the petal surface wax extraction of C. nitens Oliv,and its petal surface wax content during the full bloom period was about 1 034. 33 μg/cm2. The treatment with chloroform of 60℃ for 20 s were optimal both for the samples of C. praecox ＇Jinbei＇ and of C. praecox ＇Yangzhouhuang＇. Their petal surface wax content during the full bloom period were about 651.33 μg/cm2 extracted from C. praecox （L.）Link and 1 057. 33μg/cm2 from C. praecox ＇Yangzhouhuang＇.
出处
《北方园艺》
CAS
北大核心
2013年第23期96-99,共4页
Northern Horticulture
基金
国家自然科学基金资助项目(31272207
30800762)
关键词
氯仿法
蜡梅
花被片
蜡质含量
chloroform method
Chirnonanthus nitens
petal
wax content
