摘要
通过比较4种不含卵黄的犬精液稀释保存液在低温环境中(4℃)的实际使用效果,筛选出适合的不含卵黄低温稀释保存配方。不含卵黄的配方Ⅳ(Tris 2.108 g,柠檬酸水合物1.2 g,果糖1.0 g,青霉素100 mg,双氢链霉素100 mg,牛血清白蛋白(BSA)4 g,加蒸馏水到100 mL)具有较好的低温稀释保存效果,并首次提出牛血清白蛋白(BSA)可作为非常时期犬精液低温稀释液中卵黄的替代物。
After compared the actual use effect of four kinds of non egg yolk extenders in low temperature (4 ~C ), the suitable extenders in low temperature (4℃) which include non egg yolk was chosen.The results showed that,the non egg yolk extender IV (Tris 2.108g, Citric acid1.2g, fructose lg, penicillin(100mg), dihydrostreptomycin(100mg), bovine serum albumin(BSA) 4g,add distilled water to 100mL)had better preservation effect in low temperature,and first proposed that bovine serum albumin(BSA) could replace the egg yolk in preserving beagle canine semen in emergency time.
出处
《广东畜牧兽医科技》
2013年第6期37-40,共4页
Guangdong Journal of Animal and Veterinary Science
基金
广东省科技计划项目(2011B060300001
2011B020306008)