摘要
研究促甲状腺激素(TSH)和 Carbachol(Cch)对猪甲状腺细胞 Ca^(2+)的动员作用的结果表明,0.01～10mU/ml TSH 或10^(-5)～10^(-4)mol/L Cch 可引起甲状腺细胞[Ca^(2+)]急性升高.甲状腺细胞对 TSH 或 Cch 反应的敏感性在贴壁的单层细胞高于细胞悬液,在近汇合单层细胞高于汇合单层细胞。TSH 和 Cch 均能在无钙/EGTA 缓冲介质中刺激猪甲状腺细胞[Ca^(2+)]升高,提示此升高的[Ca^(2+)]源自细胞内储藏 Ca^(2+)的动员。
The effects of thyrotrophin(TSH)and carbachol(Cch)on intracellular free calcium concentration ([Ca^(2+)]_1)in primary cultures of porcine thyroid cells were examined.TSH and Cch caused an acute increase in[Ca^(2+)]_1 in porcine cells.The effect was mediated by the respective receptor.Responses were seen at concentration of TSH between 0.01 and 10 mU/ml,and at concentration of Cch between 10^(-5) and 10^(-4)mol/L.The sensitivity to TSH or Cch was greater in adherent monolayers of cells than that of cell suspensions and was also greater in subconfluent monolayers of cells than that of confluent ones. Both TSH and Cch were able to transiently increase[Ca^(2+)]_1 in Ca^(2+)free/EGTA buffer,indicating that the increased[Ca^(2+)]_1 originated in the mobilization of[Ca^(2+)]_1 stored in the cells.
关键词
甲状腺细胞
钙
TSH
CARBACHOL
thyrotrophin
carbachol
intracellular free calcium
thyroid cell
pig
